Publications by authors named "Ane Quesada-Ganuza"
Article Synopsis
- CRISPR-Cas9, particularly from Streptococcus pyogenes, is crucial for bacterial immunity but its evolutionary history is not well understood.!
- This study explores the evolution of Cas9 by resurrecting ancient nucleases from extinct firmicutes that existed 2.6 billion years ago and finds they had more flexible RNA and DNA target requirements compared to today's versions.!
- The research reveals that ancient Cas9 enzymes showed a gradual shift in function from nicking to creating double-strand breaks and were effective in editing both DNA and RNA, including in human cells, highlighting an evolutionary path to modern enzymes.
Background: PrsA is an extracytoplasmic folding catalyst essential in Bacillus subtilis. Overexpression of the native PrsA from B. subtilis has repeatedly lead to increased amylase yields.
View Article and Find Full Text PDFThe field of systems biology has been rapidly developing in the past decade. However, the data produced by "omics" approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the present study, a novel approach for large-scale absolute quantification of this challenging subset of proteins has been established and evaluated using osmotic stress management in the Gram-positive model bacterium as proof-of-principle precedent.
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