Evolution of antimicrobial resistance in biofilm treated with high doses of ciprofloxacin.

The evolution of antimicrobial resistance (AMR) has mainly been studied in planktonic bacteria exposed to sub-inhibitory antimicrobial (AM) concentrations. However, in a number of infections that are treated with AMs the bacteria are located in biofilms where they tolerate high doses of AM. In the present study, we continuously exposed biofilm residing at body temperature to high ciprofloxacin (CIP) concentrations increasing from 4 to 130 times the minimal inhibitory concentration (MIC), i.

Evaluation of Disinfectant Efficacy against Biofilm-Residing Wild-Type from the Porcine Industry.

is a causative pathogen of Salmonellosis, a zoonosis causing global disease and financial losses every year. Pigs may be carriers of and contribute to the spread to humans and food products. may persist as biofilms.

The Role of Biofilms in the Pathogenesis of Animal Bacterial Infections.

Biofilms are bacterial aggregates embedded in a self-produced, protective matrix. The biofilm lifestyle offers resilience to external threats such as the immune system, antimicrobials, and other treatments. It is therefore not surprising that biofilms have been observed to be present in a number of bacterial infections.

Development and validation of a regression model for Listeria monocytogenes growth in roast beefs.

Food business operators are responsible for food safety and assessment of shelf lives for their ready-to-eat products. For assisting them, a customized software based on predictive models, ListWare, is being developed. The aim of this study was to develop and validate a predictive model for the growth of Listeria monocytogenes in sliced roast beef.

The Effect of Disinfectants on Quinolone Resistant (QREC) in Biofilm.

The aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant (QREC) strains with three different biofilm matrix compositions were included to assess the log colony forming units (CFU) reduction effect of three disinfectants at various exposure times on biofilm of different ages and morphotypes. Biofilm was formed on stainless steel coupons for two and five days before transferred to tubes with Virocid 0, 25%, VirkonS 1%, and TP990 1% and left for various exposure times.

Biofilm forming properties of quinolone resistant Escherichia coli from the broiler production chain and their dynamics in mixed biofilms.

Background: Quinolone resistant Escherichia coli (QREC) have been found in samples from Norwegian broiler chicken, despite quinolones not being administered to poultry in Norway. Biofilm production may be one factor contributing to the observed persistence in the broiler production chain. In the present study, 158 QREC strains from chicken caecal and retail meat samples were screened for biofilm production in microtiter plates, biofilm morphotype on Congo Red (CR) agar plates and phylotype by multiplex PCR.

Clonal diversity and biofilm-forming ability of methicillin-resistant Staphylococcus pseudintermedius.

Objectives: The aim of this study was to investigate the clonal epidemiology of methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates from dogs in Norway and to evaluate the antimicrobial resistance patterns and determine the biofilm-forming abilities of the isolates.

Methods: All MRSP index isolates from each MRSP-positive dog detected in Norway until June 2011 were included (n = 23). The MICs of antimicrobial agents were determined by the VetMIC™ microdilution method.