In vitro shearing force testing of two seventh generation self-etching primers.

Objective: The purpose of this study was to evaluate the in vitro shearing force performance of orthodontic attachments using two self-etching primers (SEPs): iBOND and G-Bond.

Design: In vitro, laboratory study.

Material And Methods: One hundred and eighty human molars were randomly divided into four groups and again into three sub-groups with 15 teeth each.

Induction of de novo subcortical actin filament assembly by Treponema denticola major outer sheath protein.

Treponema denticola and its major outer sheath protein (Msp) induce actin reorganization in fibroblasts. We adapted a barbed-end labeling/imaging assay to monitor Msp-induced subcortical actin filament assembly in neutrophils and fibroblasts. Msp, at an actin-reorganizing concentration, inhibited migration of these dissimilar cell types, whose cytoskeletal functions in locomotion and phagocytosis are crucial for immunity and healing of peripheral infections.