Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes.

Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections.

Anthelmintic action of eprinomectin in lactating Anglo-Nubian goats in Brazil.

Eprinomectin is the only avermectin approved for use to control gastrointestinal nematodes in lactating cows. Some studies in the USA and Europe have also demonstrated that this drug is highly effective in small ruminants. The aim of the present work was to evaluate the anthelmintic efficacy of pour-on eprinomectin in Anglo-Nubian goats at the end of lactation.