DLgram cloud service for deep-learning analysis of microscopy images.

To analyze images in various fields of science and technology, it is often necessary to count observed objects and determine their parameters. This can be quite labor-intensive and time-consuming. This article presents DLgram, a universal, user-friendly cloud service that is developed for this purpose.

5-Substituted 1,2,4-Triazole-3-Carboxylates and 5-Substituted Ribavirin Analogs Synthesis.

Ribavirin analogs substituted at position 5 of the heterocyclic base are interesting for their biological activity. This protocol describes a synthetic route to several such ribavirin analogs with a wide range of substituents.© 2021 Wiley Periodicals LLC.

Nanoparticle Recognition on Scanning Probe Microscopy Images Using Computer Vision and Deep Learning.

Identifying, counting and measuring particles is an important component of many research studies. Images with particles are usually processed by hand using a software ruler. Automated processing, based on conventional image processing methods (edge detection, segmentation, etc.

5-alkylvinyl-1,2,4-triazole nucleosides: Synthesis and biological evaluation.

Some 5-substituted ribavirin analogues have a high antiviral and anticancer activity, but their mechanisms of action are obviously not the same as their parent compound. The SAR studies performed on 3 (5)-substituted 1,2,4-triazole nucleosides have shown a high dependency between the structure of the 3 (5)-substituent and the level of antiviral/anticancer activity. The most active substances of the row contain coplanar with the 1,2,4-triazole ring aromatic substituent which is connected by a rigid ethynyl bond.

Chemical ribosylation of 5-substituted 1,2,4-triazole-3-carboxylates.

The chemical ribosylation pathways of 5-substituted-1,2,4-triazole-3-carboxylates are discussed. For the products of the chemical synthesis of the 3(5)-alkyl- or 3(5)-aryl-substituted ribavirin analogues the anomer configuration and isomer composition were determined.

Isosteric ribavirin analogues: Synthesis and antiviral activities.

The novel isosteric ribavirin analogues were synthesized by two different ways. Some of them showed significant antiviral action against hepatitis C virus (HCV), herpes simplex (HCV-1) and influenza A virus comparable to that of ribavirin itself. The data obtained confirm the proposed theory of the ribavirin possible antiviral activity mechanism related with bioisosterism.

An alternative route to the arylvinyltriazole nucleosides.

A new pathway to synthesis of arylvinyl ribavirin analogues is developed which makes it possible to obtain not only trans- but also cis-isomers at vinyl bond. By this route eight ribavirin 5-arylvinyl analogues are synthesized and their antiviral activity is evaluated.