Publications by authors named "Andrey V Loskutov"
Article Synopsis
- * A new method using genetic immunization in mice successfully created antibodies targeting 71% of tested membrane proteins, confirming the potential of this DNA-gold micronanoplex technique.
- * Researchers also developed an easy in vitro method for expressing and capturing membrane proteins to assist in antibody characterization, paving the way for more research on important therapeutic membrane proteins.
The capA gene (FTT0807) from Francisella tularensis subsp. tularensis SCHU S4 encodes a 44.4 kDa integral membrane protein composed of 403 amino acid residues that is part of an apparent operon that encodes at least two other membrane proteins, CapB, and CapC, which together play a critical role in the virulence and pathogenesis of this bacterium.
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- There is a growing need for affordable and scalable gene assembly technologies to meet the demand for synthetic genes.
- The authors present a new protocol that allows for high-quality gene assembly directly from low-cost, low-quality microarray-synthesized oligonucleotides, eliminating previous purification steps through hybridization.
- Testing shows that genes assembled with this new method have comparable quality and performance to those made from more expensive, high-purity oligonucleotides, achieving a cost of about 5¢ per base.