Phenotypic and Genomic Characterization of a Sulfate-Reducing Bacterium sp. nov. Isolated from a Petroleum Reservoir in Russia.

The search for the microorganisms responsible for sulfide formation and corrosion of steel equipment in the oil fields of Tatarstan (Russia) resulted in the isolation of a new halotolerant strictly anaerobic sulfate-reducing bacterium, strain 5S69. The cells were motile curved Gram-negative rods. Optimal growth was observed in the presence of 2.

A Novel View on the Taxonomy of Sulfate-Reducing Bacterium '' and a Description of a New Species sp. nov.

Two thermophilic spore-forming sulfate-reducing strains, 435 and 781, were isolated from oil and gas reservoirs in Western Siberia (Russia) about 50 years ago. Both strains were found to be neutrophilic, chemoorganotrophic, anaerobic bacteria, growing at 45-70 °C (optimum, 55-60 °C) and with 0-4.5% (/) NaCl (optimum, 0.

Genome Analysis and Potential Ecological Functions of Members of the Genus from Subsurface Environments and Description of sp. nov.

The current work deals with genomic analysis, possible ecological functions, and biotechnological potential of two bacterial strains, HO-A22 and SHC 2-14, isolated from unique subsurface environments, the Cheremukhovskoe oil field (Tatarstan, Russia) and nitrate- and radionuclide-contaminated groundwater (Tomsk region, Russia), respectively. New isolates were characterized using polyphasic taxonomy approaches and genomic analysis. The genomes of the strains HO-A22 and SHC 2-14 contain the genes involved in nitrate reduction, hydrocarbon degradation, extracellular polysaccharide synthesis, and heavy metal detoxification, confirming the potential for their application in various environmental biotechnologies.

Genome analysis of Pollutimonas subterranea gen. nov., sp. nov. and Pollutimonas nitritireducens sp. nov., isolated from nitrate- and radionuclide-contaminated groundwater, and transfer of several Pusillimonas species into three new genera Allopusillimonas, Neopusillimonas, and Mesopusillimonas.

Two facultatively anaerobic, chemoorganoheterotrophic bacterial strains, designated JR1/69-2-13 and JR1/69-3-13, were isolated from nitrate- and radionuclide-contaminated groundwater (Ozyorsk town, South Urals, Russia). Both strains were found to be motile, Gram-stain negative rod-shaped neutrophilic, psychrotolerant bacteria that grow within the temperature range from 5-10 to 33 °C at 0-3 (0-5)% NaCl (w/v). The major cellular fatty acids were identified as C, C ω7c, C ω7c and C cyclo.

Correction: Semenova et al. Physiological and Genomic Characterization of sp. nov. from Oil-Degrading Methanogenic Enrichment and Reclassification of the Family 2022, 378.

The authors wish to make the following correction to this paper [...

Physiological and Genomic Characterization of sp. nov. from Oil-Degrading Methanogenic Enrichment and Reclassification of the Family .

The goal of the present work was to determine the diversity of prokaryotes involved in anaerobic oil degradation in oil fields. The composition of the anaerobic oil-degrading methanogenic enrichment obtained from an oil reservoir was determined by 16S rRNA-based survey, and the facultatively anaerobic chemoorganotrophic bacterial strain HO-Ch2 was isolated and studied using polyphasic taxonomy approach and genome sequencing. The strain HO-Ch2 grew optimally at 28 °C, pH 8.

sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus .

A methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera , , , , , and . A novel gram-positive, rod-shaped, anaerobic fermentative bacterium, strain 1933P, was isolated from this enrichment and characterized.

Draft Genome Sequence of Halomonas titanicae Strain TAT1, a Hydrocarbon-Oxidizing Halophilic Bacterium Isolated from a Petroleum Reservoir in Russia.

The draft genome sequence of a moderately halophilic bacterium, strain TAT1, isolated from production water of the Romashkinskoe oilfield (Russia) is presented. The genome is annotated for elucidation of the metabolic pathways involved in hydrocarbon degradation and nitrate reduction in petroleum-contaminated hypersaline environments.

Draft genome sequence data and analysis of sp. strain JR1-6 isolated from nitrate- and radionuclide-contaminated groundwater in Russia.

sp. strain JR1-6 is a Gram-negative, facultatively anaerobic, non-spore-forming, motile, rod-shaped bacterium isolated from radionuclide- and nitrate-contaminated groundwater. This bacterium reduces nitrate to N2.

Draft Genome Sequence of Geotoga petraea Strain HO-Geo1, Isolated from a Petroleum Reservoir in Russia.

The draft genome sequence of strain HO-Geo1, a bacterium isolated from production water of the Vostochno-Anzirskoe petroleum reservoir in Russia, is presented. The genome of strain HO-Geo1 is annotated for elucidation of the metabolic potential and its possible function in the subsurface microbial community and biotechnological application.

Draft Genome Sequence of a Fermenting Bacterium, sp. Strain 1933P, Isolated from a Petroleum Reservoir in Azerbaijan.

The draft genome sequence of a mesophilic fermenting bacterium, sp. strain 1933P, isolated from production water of the Binagady petroleum reservoir (Republic of Azerbaijan), is presented. The genome is annotated for elucidation of the metabolic potential and taxonomic position of strain 1933P.

sp. nov., a thermophilic bacterium isolated from a high-temperature heavy oil reservoir in China.

A rod-shaped, spore-forming, thermophilic, chemoorganotrophic, aerobic or facultatively anaerobic bacterial strain, 1017, was isolated from production water sampled at the Dagang oilfield (PR China), and was characterized by using a polyphasic approach. The strain is capable of anaerobic glucose fermentation. Nitrate is reduced to nitrite.

Draft Genome Sequence of a Fermenting Bacterium, "" 4-11, from a Low-Temperature Petroleum Reservoir in Russia.

The draft genome sequence of an anaerobic fermenting bacterium, "" strain 4-11, isolated from formation water of a low-temperature petroleum reservoir in Russia is presented. The genome is annotated to elucidate the taxonomic position of the strain 4-11 and to extend the public genome database.

Whole-genome sequence data and analysis of type strains ' and ' isolated from nitrate- and radionuclide-contaminated groundwater in Russia.

Two strains, ' JR1/69-2-13 and ' JR1/69-3-13, of aerobic, motile, Gram-negative, non-spore-forming, organotrophic, psychrotolerant bacteria were isolated from a sample of nitrate- and radionuclide-contaminated groundwater in Russia. Here we describe the draft genomes of these strains. The sequenced and annotated genome of the strain JR1/69-2-13 contained 4.

Microbial Community and Bioremediation of Groundwater by Nitrate Removal in the Zone of a Radioactive Waste Surface Repository.

The goal of the present work was to investigate the physicochemical and radiochemical conditions and the composition of the microbial community in the groundwater of a suspended surface repository for radioactive waste (Russia) and to determine the possibility of groundwater bioremediation by removal of nitrate ions. Groundwater in the repository area (10-m depth) had elevated concentrations of strontium, tritium, nitrate, sulfate, and bicarbonate ions. High-throughput sequencing of the V3-V4/V4 region of the 16S rRNA gene revealed the presence of members of the phyla (genera , and uncultured ), (genera and ), and ( Planktophila, ).

Draft Genome Sequence of Roseomonas aestuarii Strain JR1/69-1-13 Isolated from Nitrate- and Radionuclide-Contaminated Groundwater in Russia.

The draft genome sequence of strain JR1/69-1-13, an aerobic chemoorganotrophic bacterium isolated from nitrate- and radionuclide-contaminated groundwater in Russia, is presented here. The genome was annotated to elucidate the genomic basis for the strain's adaptation to the environment and its resistance to nitrate, heavy metals, and metalloids.

Diversity of Metabolically Active in Water-Flooded High-Temperature Heavy Oil Reservoir.

The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations. At this water-flooded oilfield, the microbial community of formation water from the near-bottom zone of an injection well where the most active microbial processes of oil degradation occur was investigated using molecular, cultural, radiotracer, and physicochemical techniques. The samples of microbial DNA and RNA from back-flushed water were used to obtain the clone libraries for the 16S rRNA gene and cDNA of 16S rRNA, respectively.

Genome sequencing and annotation of sp. 1017, a hydrocarbon-oxidizing thermophilic bacterium isolated from a heavy oil reservoir (China).

The draft genome sequence of sp. strain 1017, a thermophilic aerobic oil-oxidizing bacterium isolated from formation water of the Dagang high-temperature oilfield, China, is presented here. The genome comprised 3.

Draft Genome Sequence of Geobacillus subterraneus Strain K, a Hydrocarbon-Oxidizing Thermophilic Bacterium Isolated from a Petroleum Reservoir in Kazakhstan.

The draft genome sequence of Geobacillus subterraneus strain K, a thermophilic aerobic oil-oxidizing bacterium isolated from production water of the Uzen high-temperature oil field in Kazakhstan, is presented here. The genome is annotated for elucidation of the genomic and phenotypic diversity of thermophilic alkane-oxidizing bacteria.

Draft Genome Sequence of Aeribacillus pallidus Strain 8m3, a Thermophilic Hydrocarbon-Oxidizing Bacterium Isolated from the Dagang Oil Field (China).

The draft genome sequence of Aeribacillus pallidus strain 8m3, a thermophilic aerobic oil-oxidizing bacterium isolated from production water from the Dagang high-temperature oil field, China, is presented here. The genome is annotated to provide insights into the genomic and phenotypic diversity of the genus Aeribacillus.

Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity.

We report here on the in vitro and in silico characterization of the organization of the human complexin 2 (CPLX2) gene. This encodes for a protein of 134 amino acid residues, contains five exons, is localized on human chromosome 5q35.3, and spans more than 87 kb.

Distinct organization of the candidate tumor suppressor gene RFP2 in human and mouse: multiple mRNA isoforms in both species- and human-specific antisense transcript RFP2OS.

In the present study, we describe the human and mouse RFP2 gene structure, multiple RFP2 mRNA isoforms in the two species that have different 5' UTRs and a human-specific antisense transcript RFP2OS. Since the human RFP2 5' UTR is not conserved in mouse, these findings might indicate a different regulation of RFP2 in the two species. The predicted human and mouse RFP2 proteins are shown to contain a tripartite RING finger-B-box-coiled-coil domain (RBCC), also known as a TRIM domain, and therefore belong to a subgroup of RING finger proteins that are often involved in developmental and tumorigenic processes.

A novel IVS2 -2A>T splicing mutation in the GH-1 gene in familial isolated growth hormone deficiency type II in the spectrum of other splicing mutations in the Russian population.

Isolated GH deficiency (IGHD) is characterized by genetic heterogeneity, both in familial and sporadic cases. To determine if this statement can be applied to the Russian population, we performed screening for mutations in the GH-1 gene in children living in Russia with IGHD. Twenty-eight children from 26 families with total IGHD were studied.