Central effects of ethanol interact with endogenous mu-opioid activity to control isolation-induced analgesia in maternally separated infant rats.

Endogenous opioid activity plays an important role in ethanol consumption and reinforcement in infant rats. Opioid systems are also involved in mediation and regulation of stress responses. Social isolation is a stressful experience for preweanling rats and changes the effects of ethanol through opioid-dependent mechanisms.

Access to syringes for HIV prevention for injection drug users in St. Petersburg, Russia: syringe purchase test study.

Background: The HIV epidemic in Russia is concentrated among injection drug users (IDUs). This is especially true for St. Petersburg where high HIV incidence persists among the city's estimated 80,000 IDUs.

PBOV1 is a human de novo gene with tumor-specific expression that is associated with a positive clinical outcome of cancer.

PBOV1 is a known human protein-coding gene with an uncharacterized function. We have previously found that PBOV1 lacks orthologs in non-primate genomes and is expressed in a wide range of tumor types. Here we report that PBOV1 protein-coding sequence is human-specific and has originated de novo in the primate evolution through a series of frame-shift and stop codon mutations.

μ-Opioid blockade reduces ethanol effects on intake and behavior of the infant rat during short-term but not long-term social isolation.

Numerous findings in adult and infant rats have shown that the endogenous opioid system is involved in control of ethanol consumption and its reinforcing effects. Opioid systems are also involved in reactivity to social isolation with several factors (age, duration, and type of isolation) affecting this modulation. The present study investigated the effects of a selective mu-opioid antagonist CTOP (0, 0.

The role of social isolation in ethanol effects on the preweanling rat.

The present experiments investigated the effects of acute ethanol exposure on voluntary intake of 0.1% saccharin or water as well as behavioral and nociceptive reactivity in 12-day-old (P12) rats exposed to differing levels of isolation. The effects of ethanol emerged only during short-term social isolation (STSI) with different patterns observed in males and females and in pups exposed to saccharin or water.

Pharmacological effects of ethanol on ingestive behavior of the preweanling rat.

The present study was designed to test the hypothesis that sensitivity of ingestive behavior of infant rat to the pharmacological effects of ethanol changes between postnatal (P) days 9 and 12. The intake of 0.1% saccharin and water, general motor activity, and myoclonic twitching activity were assessed following administration of three doses of ethanol (0, 0.

Ethanol, saccharin, and quinine: early ontogeny of taste responsiveness and intake.

Background: Rat pups demonstrate high levels of immediate acceptance of ethanol during the first 2 weeks of postnatal life. Given that the taste of ethanol is most likely perceived by infant rats as a combination of sweet and bitter, high intake of ethanol early in ontogeny may be associated with age-related enhanced responsiveness to the sweet component of ethanol taste, as well as with ontogenetic decreases in sensitivity to its bitter component. Therefore, the present study compared responsiveness to ethanol and solutions with bitter (quinine) and sweet (saccharin) taste in terms of intake and palatability across the first 2 weeks of postnatal life.

Taste differentiation in the context of suckling and independent, adultlike ingestive behavior.

The present study compared intake of sweet (saccharin), bitter (quinine), and neutral (water) tastants available either in the context of suckling behavior through a surrogate nipple or independent adultlike feeding through an intraoral cheek cannula in 3-hr-old newborn rats lacking any suckling experience and 24-hr-old rats with regular experience with the dam's nipple. The new technique of online monitoring of fluid flow was applied for assessment of the temporal patterns of ingestion. Newborn and 1-day-old rats tested in the context of suckling behavior showed extremely low intake of quinine, moderate intake of water, and high intake of saccharin.

Repetitive exposures to a surrogate nipple providing nutritive and non-nutritive fluids: effects on suckling behavior of the newborn rat.

Responsiveness to a surrogate nipple providing water, 0.1% saccharin, 10% sucrose, pedialyte, or milk was tested in naïve-to-suckling newborn rats during six 10-min exposures, one every 1.5 h over a 7.

Oral compression activity on a surrogate nipple in the newborn rat: nutritive and nonnutritive sucking.

Newborn rats, 3 hr after birth and before any experience in suckling, were exposed for 10 min to a surrogate nipple providing milk. One hour later, they were exposed to an empty nipple for another 10-min period. The basic characteristics of oral behavior (oral compression activity, OCA) were assessed by recording intranipple pressure during the pups' first attachment to a nipple.