A new facile and fast approach to the synthesis of polyaniline (PANi) molecularly imprinted polymers (MIPs) based on aniline oxidative chemical polymerization was proposed for protein recognition. For the first time, a surface imprinting strategy was implemented for the synthesis of PANi MIPs on the inner surface of soft glass polycapillaries (PC) with a large (2237) number of individual microcapillaries. Two different PANi layers-(i) PANi film and (ii) protein imprinted PANi nanowires-were synthesized sequentially.
View Article and Find Full Text PDFMulti-channel capillaries (MC) formed from thousands individual microcapillaries with diameters ranging 10-100 μm are of a great interest for their use as platforms for molecular imprinting due to their relatively large surface area, high mechanical stability and possibility of facile integration in sensor systems. The manuscript proposes a new format of immunoassay based on imprinted protein immobilized on a MC inner surface modified with poly-l-lysine. The combination of the environmentally friendly, easy-to-produce and cheap recognition element with the carrier allowing to increase the assay sensitivity makes the described technique a perspective alternative for the existing screening tests.
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