Publications by authors named "Andrew Wiggins"
As we rapidly approach a post-antibiotic era, bacteriophage (phage) therapy may offer a solution for treating drug-resistant bacteria. is an emerging, multidrug-resistant pathogen that causes disease in people with cystic fibrosis, chronic obstructive pulmonary disease, and other underlying lung diseases. can survive inside host cells, a niche that can limit access to antibiotics.
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- Multi-antibiotic resistant (MAR) bacteria result in significant healthcare costs and loss of life, raising concerns about antibiotic misuse and the need for new antibiotic discovery.
- The role of mobile genetic elements (MGEs) in generating MAR through the acquisition of resistance genes is highlighted, emphasizing the need to investigate and potentially disable these elements.
- Exposure to non-antibiotic toxic metals, especially mercury, has been shown to co-select for MAR bacteria, suggesting that reducing exposure to such metals could lead to decreased MAR prevalence in public health.
Bacterial artificial chromosome (BAC) vectors enable stable cloning of large DNA fragments from single genomes or microbial assemblages. A novel shuttle BAC vector was constructed that permits replication of BAC clones in diverse Gram-negative species. The "Gram-negative shuttle BAC" vector (pGNS-BAC) uses the F replicon for stable single-copy replication in E.
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