Publications by authors named "Andrew V Garazha"
Article Synopsis
- DNA repair mechanisms are crucial for maintaining genome integrity and limiting tumor progression, but they can also help tumors survive radiation and chemotherapy treatments.
- Research analyzed 38 DNA repair pathways across nine cancer types using RNAseq data from various databases, revealing a consistent downregulation of the G2/M checkpoint pathway and low p53 pathway activity in tumors.
- The study identified that despite most DNA repair pathways being upregulated, key genes associated with the G2/M checkpoint and p53 pathways were significantly disrupted, highlighting their unique roles in cancer development.
Article Synopsis
- - OncoboxPD is an extensive database of human molecular pathways, featuring over 51,000 pathways that map out protein-protein interactions and metabolic reactions, totaling over 361,000 interactions among 64,000 molecules.
- - It categorizes pathways by biological processes and uses algorithms to annotate pathway nodes, allowing users to determine pathway activation levels based on specific RNA/protein expression profiles.
- - Users can visualize pathways as static or dynamic graphs, highlight differentially expressed nodes, and generate summary graphs that display the most significant upregulated and downregulated pathways for their comparisons.
Article Synopsis
- Inter-patient molecular heterogeneity in tumors is influencing the development and personalization of anticancer drugs, leading to varied treatment options.
- A comprehensive analysis of 4,890 tumors revealed that the molecular targets of accepted cancer drugs do not align with tumor heterogeneities across thirteen major cancer types.
- The study found that clinical recommendations for drug use correlate more with gene expression patterns than mutation patterns, highlighting opportunities for improving targeted therapies, especially in certain cancer types like kidney and ovarian cancers.
Despite the significant achievements in chemotherapy, cancer remains one of the leading causes of death. Target therapy revolutionized this field, but efficiencies of target drugs show dramatic variation among individual patients. Personalization of target therapies remains, therefore, a challenge in oncology.
View Article and Find Full Text PDFEndogenous retroviruses are mobile genetic elements hardly distinguishable from infectious, or "exogenous," retroviruses at the time of insertion in the host DNA. Human endogenous retroviruses (HERVs) are not rare. They gave rise to multiple families of closely related mobile elements that occupy ~8% of the human genome.
View Article and Find Full Text PDFResponses to human cytomegalovirus (HCMV) infection are largely individual and cell type specific. We investigated molecular profiles in 2 primary cell cultures of human fibroblasts, which are highly or marginally sensitive to HCMV infection, respectively. We screened expression of genes and microRNAs (miRs) at the early (3 hours) stage of infection.
View Article and Find Full Text PDFBackground: MicroRNAs (miRNAs) are a class of small RNAs that regulate gene expression. They are aberrantly expressed in many human cancers and are potential therapeutic targets and molecular biomarkers.
Methods: In this study, we for the first time validated the reported data on the entire set of published differential miRNAs (102 in total) through a series of transcriptome-wide experiments.
MicroRNAs (miRNAs) are small, non-coding RNAs that post-transcriptionally regulate gene expression. Their altered expression and functional activity have been observed in many human cancers. miRNAs represent promising diagnostic and prognostic molecular biomarkers, and also serve as novel therapeutic targets.
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