Publications by authors named "Andrew T Dennis"
Biochemistry
October 2006
Article Synopsis
- Calcium-saturated calmodulin (CaM) is crucial in cells for binding various target proteins in calcium-dependent signaling pathways but often causes issues for calcium biosensors due to unwanted interactions with other proteins.
- To enhance CaM-based sensors, researchers aimed to create modified CaM and target protein pairs that maintain their affinity but do not react with the native CaM and its usual partners.
- By using computational design methods and experimental analysis, they successfully identified specific mutations in both CaM and a peptide (M13) that allowed for unique binding, achieving similar affinity to the original complex while reducing cross-reactivity.
