Secondary transport mechanisms in amino acid fed enhanced biological phosphorus removal.

Enhanced biological phosphorus removal (EBPR) water resource recovery facilities (WRRFs) often fail to meet phosphorus discharge permit limits, indicating a need to improve EBPR to reduce environmental phosphorus discharges. EBPR designs are largely based on the Accumulibacter polyphosphate accumulating organism (PAO) metabolism, while understudied Tetrasphaera PAOs are equally important to EBPR in many facilities worldwide. Anaerobic organic carbon competition is believed to be a key driver of EBPR reliability.

Formylpeptide receptor 1 contributes to epidermal barrier dysfunction-induced skin inflammation through NOD-like receptor C4-dependent keratinocyte activation.

Background: Skin barrier dysfunction may both initiate and aggravate skin inflammation. However, the mechanisms involved in the inflammation process remain largely unknown.

Objectives: We sought to determine how skin barrier dysfunction enhances skin inflammation and molecular mechanisms.

Effects of media length on biofilms and nitrification in moving bed biofilm reactors.

Biofilms grown on free-floating plastic media are increasingly being used to cultivate biofilms in integrated fixed film activated sludge (IFAS) and moving bed bioreactor (MBBR) systems for wastewater treatment with the common goal of increasing nitrogen removal. Fundamental principles of fluid dynamics dictate that the length of internal media channels affects fluid velocities and shear forces across biofilm surfaces, which in turn should affect rates of mass transfer and biofilm growth and activity, but little is known about media length effects on water quality and biofilm characteristics. It was hypothesized that length affects biofilm thickness, microbial populations and their activities, and system performance.

Effects of Methyl, Ester, and Amine Surface Groups on Microbial Activity and Communities in Nitrifying Biofilms.

The objective of this study was to determine how different attachment surface chemistries affected the initial and long-term performance and microbial populations of nitrifying biofilms under well-controlled hydrodynamic mixing conditions. While much previous research has focused on the effects of surface properties such as hydrophobicity on bacterial attachment in pure cultures, this study evaluated the effects of specific functional groups on mixed culture composition and functional behavior. Three surfaces with varying hydrophobicity and charge were evaluated for biofilm community development and performance: unmodified poly(dimethylsiloxane) (PDMS), which included terminal methyl groups and was relatively hydrophobic (P-Methyl), PDMS silanized with ester groups (P-Ester), which was uncharged and relatively hydrophilic, and PDMS modified with amine groups (P-Amine), which possessed a positive charge and was the most hydrophilic.

Symmetric drug-related intertriginous and flexural exanthema: Clinicopathologic study of 19 cases and review of literature.

Background: Symmetric drug-related intertriginous and flexural exanthema (SDRIFE) is a cutaneous drug reaction characterized by gluteal/anogenital erythema and symmetric involvement of other intertriginous location(s) without systemic signs. Clinicopathologic characterization has been limited to case reports and small series. We describe 19 new cases and review the literature to better define the clinical and histopathologic spectrum of SDRIFE.

Effects of surface skewness on local shear stresses, biofilm activity, and microbial communities for wastewater treatment.

This study's objective was to assess attachment surface skewness (asymmetric surface height variation) effects on biofilm development. 3D printed molds were used to create surfaces with 300 μm features to provide opposite skewness but identical roughness values. Surfaces with negative skewness had consistently greater nitrite oxidation and biomass growth than other surfaces during biofilm development when studied in annular bioreactor systems.

Folliculitis Induced by Laser Hair Removal: Proposed Mechanism and Treatment.

The neodymium:aluminum garnet laser has emerged as a generally well-tolerated tool for hair removal; however, some patients develop a folliculitis after treatment, which can limit utility. To our knowledge, the literature is currently lacking an adequate description of the etiology of laser-induced folliculitis or strategies to prevent and manage it. We present the case of a 33-year-old Caucasian male patient who developed a robust laser-induced folliculitis.

IFN-γ enhances cell-mediated cytotoxicity against keratinocytes via JAK2/STAT1 in lichen planus.

Lichen planus (LP) is a chronic debilitating inflammatory disease of unknown etiology affecting the skin, nails, and mucosa with no current FDA-approved treatments. It is histologically characterized by dense infiltration of T cells and epidermal keratinocyte apoptosis. Using global transcriptomic profiling of patient skin samples, we demonstrate that LP is characterized by a type II interferon (IFN) inflammatory response.

Battling Neuropathic Scar Pain With Botulinum Toxin.

Botulinum toxin type A (BTA) is a neurotoxic protein that prevents the release of neurotransmitters from presynaptic nerves and has shown promise in treating neuropathic pain. Recently, BTA has been used to treat painful keloids and scars. We present a patient with refractory neuropathic pain in a normotrophic spread-scar treated with the injection of BTA.

A Case of Adjacent, Clonally Distinct Borderline Melanocytic Tumors on the Arm.

Atypical Spitz tumor (AST) is a melanocytic proliferation that shares histopathologic features of Spitz nevus and spitzoid melanoma. Distinction of AST from spitzoid melanoma is critical because the majority of ASTs will follow an indolent course. Array-based comparative genomic hybridization (aCGH) has been suggested as a potential tool for evaluating malignant potential in spitzoid tumors.

Performance and diversity responses of nitrifying biofilms developed on varied materials and topographies to stepwise increases of aeration.

Nitrifying biofilms were grown on 3D-printed nylon with three different surface characteristics (flat, millimeter-scale indentations, and indentations with activated carbon (AC) coating) and were subjected to sequentially increasing aeration-based shear to determine the interplay between surface, performance, and microbial populations towards improved design of wastewater treatment media. Biofilms were evaluated for nitrification, biomass detachment, and microbial composition based on Illumina 16s rRNA sequencing. Indentations provided greater stability over flat with respect to population diversity after detachment events but did not improve ammonia removal.

Psammomatous Squamous Cell Carcinoma of the Skin.

Psammoma bodies (PBs) are concentric, lamellated calcifications commonly observed in malignancies such as papillary thyroid carcinoma and serous carcinoma of the ovary in which they may serve prognostic value. PBs are rare in cutaneous squamous cell carcinoma (cSCC), with only 1 previously reported case. Here, we present 3 cases of cSCC displaying PBs.

Magnetic orientation in C. elegans relies on the integrity of the villi of the AFD magnetosensory neurons.

The magnetic field of the earth provides many organisms with sufficient information to successfully navigate through their environments. While evidence suggests the widespread use of this sensory modality across many taxa, it remains an understudied sensory modality. We have recently showed that the nematode C.

Methods for increasing the rate of anammox attachment in a sidestream deammonification MBBR.

Deammonification (partial nitritation-anammox) is a proven process for the treatment of high-nitrogen waste streams, but long startup time is a known drawback of this technology. In a deammonification moving bed biofilm reactor (MBBR), startup time could potentially be decreased by increasing the attachment rate of anammox bacteria (AMX) on virgin plastic media. Previous studies have shown that bacterial adhesion rates can be increased by surface modification or by the development of a preliminary biofilm.

Conjugated gold nanoparticles as a tool for probing the bacterial cell envelope: The case of Shewanella oneidensis MR-1.

The bacterial cell envelope forms the interface between the interior of the cell and the outer world and is, thus, the means of communication with the environment. In particular, the outer cell surface mediates the adhesion of bacteria to the surface, the first step in biofilm formation. While a number of ligand-based interactions are known for the attachment process in commensal organisms and, as a result, opportunistic pathogens, the process of nonspecific attachment is thought to be mediated by colloidal, physiochemical, interactions.

High catalytic activity and pollutants resistivity using Fe-AAPyr cathode catalyst for microbial fuel cell application.

For the first time, a new generation of innovative non-platinum group metal catalysts based on iron and aminoantipyrine as precursor (Fe-AAPyr) has been utilized in a membraneless single-chamber microbial fuel cell (SCMFC) running on wastewater. Fe-AAPyr was used as an oxygen reduction catalyst in a passive gas-diffusion cathode and implemented in SCMFC design. This catalyst demonstrated better performance than platinum (Pt) during screening in "clean" conditions (PBS), and no degradation in performance during the operation in wastewater.

Bilirubin oxidase based enzymatic air-breathing cathode: Operation under pristine and contaminated conditions.

The performance of bilirubin oxidase (BOx) based air breathing cathode was constantly monitored over 45 days. The effect of electrolyte composition on the cathode oxygen reduction reaction (ORR) output was investigated. Particularly, deactivation of the electrocatalytic activity of the enzyme in phosphate buffer saline (PBS) solution and in activated sludge (AS) was evaluated.

Influence of anode surface chemistry on microbial fuel cell operation.

Self-assembled monolayers (SAMs) modified gold anodes are used in single chamber microbial fuel cells for organic removal and electricity generation. Hydrophilic (N(CH3)3(+), OH, COOH) and hydrophobic (CH3) SAMs are examined for their effect on bacterial attachment, current and power output. The different substratum chemistry affects the community composition of the electrochemically active biofilm formed and thus the current and power output.

Relationship between surface chemistry, biofilm structure, and electron transfer in Shewanella anodes.

A better understanding of how anode surface properties affect growth, development, and activity of electrogenic biofilms has great potential to improve the performance of bioelectrochemical systems such as microbial fuel cells. The aim of this paper was to determine how anodes with specific exposed functional groups (-N(CH3)3 (+), -COOH, -OH, and -CH3), created using ω-substituted alkanethiolates self-assembled monolayers attached to gold, affect the surface properties and functional performance of electrogenic Shewanella oneidensis MR-1 biofilms. A combination of spectroscopic, microscopic, and electrochemical techniques was used to evaluate how electrode surface chemistry influences morphological, chemical, and functional properties of S.

Double-chamber microbial fuel cell with a non-platinum-group metal Fe-N-C cathode catalyst.

Non-Pt-group metal (non-PGM) materials based on transition metal-nitrogen-carbon (M-N-C) and derived from iron salt and aminoantipyrine (Fe-AAPyr) of mebendazole (Fe-MBZ) were studied for the first time as cathode catalysts in double-chamber microbial fuel cells (DCMFCs). The pH value of the cathode chamber was varied from 6 to 11 to elucidate the activity of those catalysts in acidic to basic conditions. The Fe-AAPyr- and Fe-MBZ-based cathodes were compared to a Pt-based cathode used as a baseline.