Validation of a real-time polymerase chain reaction for the detection and quantification of the nucleic acid of from equine clinical samples.

Unlabelled: var (HCF) is a dimorphic fungus that causes epizootic lymphangitis in equids. Current diagnostic approaches, including culture, microscopy, and clinical presentation, lack speed, sensitivity, and specificity when diagnosing clinical cases. In this study, equine blood and pus samples on Whatman FTA cards from Senegal ( = 3), The Gambia ( = 19), Ethiopia ( = 16), and Mali ( = 13) were tested using a real-time PCR (qPCR) protocol.

Prevalence, molecular characterization, and antimicrobial resistance profiles of Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7 on dairy cattle farms in Jordan.

This study determined the prevalence, pulsed-field gel electrophoresis profiles, and antimicrobial resistance profile of Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7 isolates from dairy cattle farms in Jordan. Samples from bulk tank milk (n = 305), cattle feces (n = 610), and rectoanal mucosal swabs (n = 610) were collected from 61 dairy cattle farms. We confirmed 32 L.

Evaluating the Efficacy of Knowledge-Transfer Interventions on Animal Health Knowledge of Rural Working Equid Owners in Central Ethiopia: A Cluster-Randomized Controlled Trial.

The objectives of this study were to evaluate the efficacy of several knowledge-transfer interventions about donkey health, utilizing a cluster-randomized controlled trial (c-RCT), on the long-term knowledge change (~6 months post intervention) of Ethiopian rural working equid owners. Knowledge transfer interventions included: an audio programme, a village meeting and a diagrammatic hand-out, which were also compared to a control group, which received no intervention. All interventions addressed identical learning objectives.

Seroprevalence, risk factors and spatial distribution of West Nile virus in Jordan.

Background: This is the first countrywide study of the seroprevalence, risk factors and spatial distribution of West Nile virus (WNV) in Jordan.

Methods: A cross-sectional sample of 801 participants was administered a comprehensive questionnaire survey and tested for WNV immunoglobulin G antibodies.

Results: The point seroprevalence rate for WNV infection was 8.

Infectious diseases of working equids.

Most working equids reside in low-income countries where they have an essential role in the livelihoods of their owners. Numerous infectious diseases negatively impact the health and productivity of these animals. There are considerable technical, social-behavioral, and institutional impediments globally to reducing the burden of infectious diseases on working equids.

Reducing sampling error in faecal egg counts from black rhinoceros (Diceros bicornis).

Faecal egg counts (FECs) are commonly used for the non-invasive assessment of parasite load within hosts. Sources of error, however, have been identified in laboratory techniques and sample storage. Here we focus on sampling error.