Efficiency Correction Is Required for Accurate Quantitative PCR Analysis and Reporting.

Background: Quantitative PCR (qPCR) aims to measure the DNA or RNA concentration in diagnostic and biological samples based on the quantification cycle (Cq) value observed in the amplification curves. Results of qPCR experiments are regularly calculated as if all assays are 100% efficient or reported as just Cq, ΔCq, or ΔΔCq values.

Contents: When the reaction shows specific amplification, it should be deemed to be positive, regardless of the observed Cq.