Management of Keratoacanthoma: 157 Tumors Treated With Surgery or Intralesional Methotrexate.

Background: Keratoacanthomas (KAs) are common tumors of squamous cell origin that grow rapidly and may regress; however, differentiation from an aggressive squamous cell carcinoma is problematic.

Objective: To report the authors' experience in managing KA with intralesional methotrexate (MTX) or surgical excision.

Materials And Methods: The authors collected data on 157 tumors (136 patients) over 6 months from a single institution.

Targeting Notch1 and IKKα Enhanced NF-κB Activation in CD133 Skin Cancer Stem Cells.

Cancer stem-like cells are hypothesized to be the major tumor-initiating cell population of human cutaneous squamous cell carcinoma (cSCC), but the landscape of molecular alterations underpinning their signaling and cellular phenotypes as drug targets remains undefined. In this study, we developed an experimental pipeline to isolate a highly enriched CD133CD31CD45CD61CD24 (CD133) cell population from primary cSCC specimens by flow cytometry. The CD133 cells show enhanced stem-like phenotypes, which were verified by spheroid and colony formation and tumor generation Gene expression profiling of CD133 cells was compared and validated, and differentially expressed gene signatures and top pathways were identified.