DeMixSC: a deconvolution framework that uses single-cell sequencing plus a small benchmark dataset for improved analysis of cell-type ratios in complex tissue samples.

Bulk deconvolution with single-cell/nucleus RNA-seq data is critical for understanding heterogeneity in complex biological samples, yet the technological discrepancy across sequencing platforms limits deconvolution accuracy. To address this, we introduce an experimental design to match inter-platform biological signals, hence revealing the technological discrepancy, and then develop a deconvolution framework called DeMixSC using the better-matched, i.e.

Perceived prevalence of peer marijuana use: changes among college students before and after Oregon recreational marijuana legalization.

: Oregon students' rates of marijuana use have increased following recreational marijuana legalization (RML), but the relation between RML and students' perceived peer use of marijuana - or descriptive norms - is unknown. : This study examined whether perceived peer use of marijuana changed more following RML for college students in Oregon than in states without RML. : Repeated cross-sectional data were collected from the National College Health Assessment-II surveys from 2008 to 2016 to assess changes in descriptive norms ("Within the last 30 days, what percent of students at your school used marijuana?") among 18-26 year-old undergraduates from two Oregon institutions and 123 institutions in non-RML states (N = 280,006; 66.

Oregon recreational marijuana legalization: Changes in undergraduates' marijuana use rates from 2008 to 2016.

There have been few studies of marijuana use before and after recreational marijuana legalization (RML) in affected states. We tested whether marijuana use rates increased more among college students in Oregon than in non-RML states following Oregon RML in July 2015. Repeated cross-sectional National College Health Assessment-II surveys were completed by random samples of students within participating colleges from 2008 to 2016.