Publications by authors named "Andrew F Seila"
Article Synopsis
- The ribosome is crucial for protein synthesis in all cells, and this study examines the kinetic effects of isotopic substitutions at specific sites within the ribosome's 50S fragment.
- A simplified assay shows that the ribosome follows a specific reaction mechanism, where the first irreversible step is the creation of a tetrahedral intermediate, with a consistent isotope effect observed regardless of pH levels.
- The findings indicate that the ribosome plays a chemical role in catalyzing peptide bond formation, differentiating its mechanism from uncatalyzed reactions occurring in solution.
