Publications by authors named "Andrew F Page"
Background: With the increasing interest in metabolic engineering of plants using genetic manipulation and gene editing technologies to enhance growth, nutritional value and environmental adaptation, a major concern is the potential of undesirable broad and distant effects of manipulating the target gene or metabolic step in the resulting plant. A comprehensive transcriptomic and metabolomic analysis of the product may shed some useful light in this regard. The present study used these two techniques with plant cell cultures to analyze the effects of genetic manipulation of a single step in the biosynthesis of polyamines because of their well-known roles in plant growth, development and stress responses.
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- Arginine and ornithine are key substrates for polyamine biosynthesis in plants and are involved in nitrogen metabolism, with their regulation being complex.
- The study employed RT-qPCR to analyze gene expression in poplar cells with enhanced polyamine production due to transgenic expression of mouse Orn decarboxylase.
- Results indicated that gene expression in the Orn/Arg biosynthetic pathway is largely stable and regulated biochemically, rather than through significant changes in gene expression in response to increased polyamine levels.
Recent years have seen a huge increase and improvement in techniques for analysis of transgene expression in plants. The analysis of RNA frequently provides a vital link between changes in enzyme levels and/or metabolites and the phenotype. This chapter focuses on RNA-based techniques for the analysis of transgene expression, beginning with the extraction of RNA and its evaluation in terms of purity and integrity by spectrophotometry and gel electrophoresis, respectively.
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