Recovery kinetics of dual AAV-mediated human otoferlin expression.

Deafness-causing deficiencies in () have been addressed preclinically using dual adeno-associated virus (AAV)-based approaches. However, timing of transduction, recombination of mRNA, and protein expression with dual hybrid AAV methods methods have not previously been characterized. Here, we have established an assay to determine the kinetics of dual-AAV mediated expression of in hair cells of the mouse utricle.