Uncovering the genetic diversity within the virome and isolation of new viruses from this highly urbanised and invasive mosquito.

The Australian backyard mosquito, , is a highly urbanised pest species that has invaded New Zealand and the USA. Importantly, has been implicated as a vector of Ross River virus, a common and arthritogenic arbovirus in Australia, and is a laboratory vector of numerous other pathogenic viruses, including West Nile, yellow fever, and Zika viruses. To further explore live viruses harboured by field populations of and, more specifically, assess the genetic diversity of its virome, we processed 495 pools, comprising a total of 6,674 female collected across fifteen suburbs in Brisbane, Australia, between January 2018 and May 2019.