The role of eukaryotic initiation factor 2alpha during the metabolic depression associated with estivation.

We have investigated the role of eukaryotic initiation factor 2alpha (eIF2alpha) in two estivating organisms previously shown to downregulate protein synthesis during metabolic depression, the land snail Helix aspersa Müller and the desert frog Neobatrachus sutor Main 1957. We have developed a method using a single antibody (which binds specifically to the phosphorylated, conserved phosphorylation region) by which the total levels of eIF2alpha and the ratio of phosphorylated eIF2alpha [eIF2alpha(P)] to total (phosphorylated and unphosphorylated) eIF2alpha can be determined. In H.

In vivo downregulation of protein synthesis in the snail Helix apersa during estivation.

Protein synthesis is downregulated during metabolic depression in a number of systems where the metabolic depression is effected by obvious extrinsic cues. The metabolic depression of the estivating land snail Helix apersa occurs in the absence of any obvious physiological stress and has an intrinsic component independent of temperature, pH, O(2) status, or osmolality. We show that this metabolic depression is accompanied by a downregulation of protein synthesis in vivo.