Publications by authors named "Andressa Vaz"
Background: Minimal residual disease (MRD) in chronic lymphocytic leukemia (CLL) has prognostic and predictive significance. One of the approaches to detect MRD by flow cytometry (FC) is the use of dry antibody reagents such as DuraClone® RE CLB (Beckman Coulter-BC). The aim of this study was to evaluate the performance of the DuraClone® RE CLB in detecting MRD in CLL compared to liquid reagents.
View Article and Find Full Text PDFObjective: To validate multilineage score system correlating results of flow cytometry, cytogenetics, cytomorphology and histology from samples of patients with suspected myelodysplastic syndrome or cytopenia of unknown origin.
Methods: A retrospective study analyzing laboratory data of 49 patients with suspected myelodysplastic syndrome or cytopenia of unknown origin, carried out between May and September 2017. The inclusion criteria were availability of flow cytometry results, and at least one more method, such as morphology, histology or cytogenetics.
Detection of Central Nervous System Infiltration by Myeloid and Lymphoid Hematologic Neoplasms Using Flow Cytometry Analysis: Diagnostic Accuracy Study.
Front Med (Lausanne)
May 2018
Article Synopsis
- Infiltration of the central nervous system (CNS) by cancerous cells can be serious and often fatal, but analyzing cerebrospinal fluid (CSF) for these cells can be challenging due to low cell counts and quick cell degradation.
- The study aimed to evaluate how effectively flow cytometry (FC) can detect CNS infiltration by cancer compared to traditional cytomorphology, using a special cell stabilizer to improve sample preservation.
- Results showed that FC was significantly more sensitive and specific than cytomorphology, especially for samples with fewer than 5 leukocytes per mm, highlighting the benefits of using a stabilizing medium for CSF collection.
Objective:: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols.
Methods:: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria.
Results:: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection.