Immunopathological studies of orthotopic human liver allografts.

Twenty-six specimens obtained from twenty human orthotopic liver allografts 10–968 days after transplantation were studied by light microscopy, electron microscopy, and immunofluorescence. The main lesions consisted of mononuclear-cell infiltration around the portal tracts, centrilobular cholestasis, liver-cell atrophy and reticulin collapse, obliterative intimal thickening of hepatic arteries, and fibrosis. Moderate amounts of IgG and/or IgM and complement (β1C/β1A globulin or C'lq) were observed in four of the liver samples and smaller deposits were present in another five.

Net histocompatibility ratios (NHR) for clinical transplantation.

In this study, the histocompatibility match of a group of patients treated at least one and a half years ago was calculated by a modification of the net histocompatibility ratio (NHR) formula of Rapaport and Dausset. Correlations were then made with the outcome as judged by patient and kidney survival, homograft function, the magnitude of maintenance immunosuppression, and the extent of histopathologic abnormalities in the transplants.

Localization of fluorescein-labeled antinucleoside antibodies in glomeruli of patients with active systemic lupus erythematosus nephritis.

Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA. The first group consisted of 13 SLE patients, and the second consisted of 53 patients with non-SLE nephropathies. The data obtained from the two groups of patients were used for comparison, and they showed the following:(a) Fl-Abs to immunoglobulins and complement were bound in the glomeruli of tissues from all patients with active SLE glomerulonephritis characterized by deposits of foreign material in glomerular capillary walls (GCW).

Thymectomy and renal homotransplantation.

The course of forty-six consecutive patients were studied for 3½–5 years after renal homotransplantation from related and unrelated donors. Transthoracic thymectomy was performed before transplantation in twenty-four cases; the other twenty-two recipients served as controls. A similar spectrum of donor-recipient lymphocyte antigen compatibility was present in both the test and control series.

Human renal transplants. 3. Immunopathologic studies.

Tissues obtained from 34 human renal allografts by biopsy, 1 to 31 months after transplantation, were studied by histologic, immunofluorescence, and immunoferritin techniques. Our purpose was to gain more precise information concerning the localization of immunoglobulins and complement in glomeruli and to describe the associated tubular and vascular changes. 1.

Immunologic studies of autoimmune disease in NZB-NZW F1 mice. I. Binding of fluorescein-labeled antinucleoside antibodies in lesions of lupus-like nephritis.

For the past 3 years NZB and NZW mice have been maintained by sister-brother matings from English breeder stock. NZB/NZW F(1) hybrids developed lupus-like nephritis during the 6th to 7th month and few survived beyond the 8th month. Renal tissues of these animals were examined with fluorescein-labeled antinucleoside sera, specific for thymine and cytosine, for the presence of denatured DNA in GCW, and with labeled antibody to mouse IgG for the presence of excess host globulin in the same areas.

Electron microscopic studies of human glomerulonephritis with ferritin-conjugated antibody. Localization of antigen-antibody complexes in glomerular structures of patients with acute glomerulonephritis.

1. Kidney biopsies from 4 cases of severe acute glomerulonephritis were obtained 11 to 25 days after the onset of clinical manifestations of the disease. These tissues were treated with ferritin-conjugated antibodies to 7S gamma-globulin, beta(1C), and Type 12 streptococcal products.