Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy.

The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A.

Respiratory alterations in workers: study of single ports.

Objective: To identify alterations in the respiratory system in port workers through radiographic and pulmonary function tests; to identify the use of personal protective equipment during port activities; and to relate age, working time, exposure to substances such as fertilizers and the use of personal protective equipment during port activities, to changes in the respiratory system in port workers.

Method: Descriptive and exploratory study, in south Brazilian maritime port, from July of 2014 to January of 2015. A retrospective quantitative analysis of the results of chest x-ray and spirometry of 695 port workers' chart and prospective analysis of 66 workers were performed.

Occupational health nurses: interdisciplinary experience in occupational health.

Objective:: to analyze the relationship of occupational health nurses with the other members of the Specialized Service in Safety Engineering and Occupational Medicine (SESMT) and characterize joint actions of these professionals in occupational health.

Method:: qualitative, exploratory, and descriptive study with 34 professionals of seven companies from the South Macroregion of the state of Rio Grande do Sul. Interviews and observations were conducted for content analysis of Bardin.

Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors.

Objective: To develop a novel and potent fusion inhibitor of HIV infection based on a rational strategy for synthetic antibody library construction.

Design: The reduced molecular weight of single-domain antibodies (sdAbs) allows targeting of cryptic epitopes, the most conserved and critical ones in the context of HIV entry. Heavy-chain sdAbs from camelids are particularly suited for this type of epitope recognition because of the presence of long and flexible antigen-binding regions [complementary-determining regions (CDRs)].

A comprehensive high-throughput FTIR spectroscopy-based method for evaluating the transfection event: estimating the transfection efficiency and extracting associated metabolic responses.

Reporter genes are routinely used in every laboratory for molecular and cellular biology for studying heterologous gene expression and general cellular biological mechanisms, such as transfection processes. Although well characterized and broadly implemented, reporter genes present serious limitations, either by involving time-consuming procedures or by presenting possible side effects on the expression of the heterologous gene or even in the general cellular metabolism. Fourier transform mid-infrared (FT-MIR) spectroscopy was evaluated to simultaneously analyze in a rapid (minutes) and high-throughput mode (using 96-wells microplates), the transfection efficiency, and the effect of the transfection process on the host cell biochemical composition and metabolism.