Flavonoid aglycones are secondary plant metabolites that exhibit a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, anticancer, and antiplatelet effects. However, the precise molecular mechanisms underlying their inhibitory effect on platelet activation remain poorly understood. In this study, we applied flow cytometry to analyze the effects of six flavonoid aglycones (luteolin, myricetin, quercetin, eriodictyol, kaempferol, and apigenin) on platelet activation, phosphatidylserine externalization, formation of reactive oxygen species, and intracellular esterase activity.
View Article and Find Full Text PDFIn this study, we expanded our previous work by testing compounds for their ability to inhibit platelet activation at low (30 µM) concentration by inhibition of ROS production, thromboxane synthase (TxS) activity, and activation of cyclic nucleotide pathways. We also investigated whether some of these compounds could potentiate the effects of P2Y12 ADP receptor inhibitor action and discussed possible structure-activity relationships of the tested compounds. We showed that at this concentration only compounds and significantly inhibited thrombin-induced platelet activation which was accompanied by inhibition of ROS production and thromboxane synthase activity.
View Article and Find Full Text PDFChemical investigation of the polypore fungus Fistulina hepatica resulted in the isolation of five compounds, including four new polyacetylenic fatty acid derivatives - isocinnatriacetin B (1), isocinnatriacetin A (2), cinna-triacetin C (3) and ethylcinnatriacetin A (4) together with one known polyacetylene fatty acid derivative - cinnatriacetin A (5). The structures were elucidated using spectroscopic methods (UV, NMR, HR-ESIMS) along with comparison to literature data. Antibacterial activity screening of compounds 1-5 against ESKAPE bacterial strains in vitro with zones of inhibition (ZOI) was performed and MIC values were established for the most active compounds (3 and 4).
View Article and Find Full Text PDFTwo previously undescribed isoquinoline alkaloids, bracteatinine (1) and isogroenlandicine (2), together with four known alkaloids - coptisine (3), dehydrocorydaline (4), palmatine (5) and jatrorrhizine (6) were isolated from the aerial parts of Corydalis bracteata (Steph. Ex. Willd.
View Article and Find Full Text PDFChemical investigation of the hydnoid fungus resulted in the isolation of five compounds, including two new sarcodontic acid derivatives - setosic acid () and 7,8-dehydrohomosarcodontic acid () along with three known benzoquinone pigments - sarcodontic acid (), 4,5-dehydrosarcodontic acid () and dihydrosarcodontic acid (). The structures were elucidated using spectroscopic methods (UV, NMR and HR-ESIMS). The biosynthetic relationship of the isolated compounds is proposed and discussed.
View Article and Find Full Text PDFThe roots of licorice ( L.) have been widely used in traditional and officinal medicines for the treatment of different diseases. Natural deep eutectic solvents (NADES) have become popular for the extraction of active principles from medicinal plants.
View Article and Find Full Text PDFWater avens ( L.) is a common plant widely spread in Europe and North America. It is rich in biologically active natural products, some of which are promising as prospective pharmaceuticals.
View Article and Find Full Text PDFOne new compound isoembinin 1 along with ten known compounds 2-11 were isolated from the terrestrial parts of Iris lactea Pall. All of the compound structures were determined through extensive 1D and 2D NMR experiments along with HR-ESIMS analysis and comparison with literature data. Because many flavonoids exert antiplatelet and antioxidant activity we tested the effects of the isolated flavone C-glycosides 1-9 on platelet activation and reactive oxygen species (ROS) production.
View Article and Find Full Text PDFTwo undescribed (- and five known cyclic diarylheptanoids (-) were isolated from the false heartwood of white birch ( Ehrh.). All structures were elucidated through extensive 1D and 2D NMR experiments and HR-ESI-MS data, along with comparison of their spectroscopic data with those reported in the literature.
View Article and Find Full Text PDFA new dihydrochalcone, 2',4'-dimethoxydihydrochalcone (), together with 7 known compounds, 2',4'-dihydroxydihydrochalcone (), 2'-hydroxy-4'-methoxydihydrochalcone (), 2'-hydroxy-4'-methoxychalcone (), 1-(3,5-dihydroxy-4-methoxyphenyl)-2-(3-hydroxyphenyl) ethane (), 2,3,4,7-tetramethoxy-9,10-dihydrophenanthrene (), 5-hydroxy-2,3,4-trimethoxy-9,10-dihydrophenanthrene () and 5,7-dihydroxy-6,8-dimethyl flavanone () were isolated from the shoots of L. The structures of these compounds were elucidated using 1D and 2D NMR experiments along with HR-ESI-MS. Compound is reported for the genus for the first time.
View Article and Find Full Text PDFThe effects of temperature, reactant ratio, pH, and reaction time were studied on the polymers formed by the reactions of succinic and glutaric anhydrides with chitosan under both homogeneous and heterogeneous conditions. As a result, protocols were developed for the synthesis of succinyl- and glutaryl-chitosan derivatives (SC and GC, respectively) with a specific degree of substitution. The polymers were characterized by NMR spectroscopy, including two-dimensional NMR techniques, that confirms N-substitution of chitosan under reaction conditions used.
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