Quantification of lysosomal labile Zn and monitoring of Zn efflux using a small-molecule-protein hybrid fluorescent probe.

Lysosomal labile Zn levels have been unclear. By targeting a small-molecule fluorescent Zn probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn concentration was determined to be 1.9 nM in HeLa cells.

Pharmaceutical Nanoparticles Formation and Their Physico-Chemical and Biomedical Properties.

The use of medicinal substances in nanosized forms (nanoforms, nanoparticles) allows the therapeutic effectiveness of pharmaceutical preparations to be increased due to several factors: (1) the high specific surface area of nanomaterials, and (2) the high concentration of surface-active centers interacting with biological objects. In the case of drug nanoforms, even low concentrations of a bioactive substance can have a significant therapeutic effect on living organisms. These effects allow pharmacists to use lower doses of active components, consequently lowering the toxic side effects of pharmaceutical nanoform preparations.

Endotoxemic acute renal failure is attenuated in caspase-1-deficient mice.

Caspase-1-deficient (-/-) mice are protected against sepsis-induced hypotension and mortality. We investigated the role of caspase-1 and its associated cytokines in a nonhypotensive model of endotoxemic acute renal failure (ARF). Mice were injected intraperitoneally with 2.