sp. nov., a Thermophilic, Obligately Chemolithoautotrophic, Sulfur-Oxidizing Bacterium from a Hot Spring and Proposal of fam. nov.

An aerobic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium, strain AK1, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka, Russia. The cells of the new isolate were Gram-negative motile rods with a single polar flagellum. Strain AK1 grew at 37-55 °C (optimum 50 °C) with 0-1.

A Novel Species of the Genus Thermanaerothrix Isolated from a Kamchatka Hot Spring Possesses Hydrolytic Capabilities.

Hot springs are inhabited by specific microbial communities which are reservoirs of novel taxa. In this work strain 4228-RoL was isolated from the Solnechny hot spring, Uzon Caldera, Kamchatka. Cells of the strain 4228-RoL were Gram-negative rods forming multicellular filaments.

gen. nov., sp. nov., a Novel Anaerobic Free-Living Mollicute Isolated from a Terrestrial Mud Volcano.

A novel aerotolerant anaerobic bacterium (strain M4Ah) was isolated from a terrestrial mud volcano (Taman Peninsula, Russia). Cells were small, cell-wall-less, non-motile cocci, 0.32-0.

Pseudodesulfovibrio pelocollis sp. nov. a Sulfate-Reducing Bacterium Isolated from a Terrestrial Mud Volcano.

Terrestrial mud volcanoes (TMVs), surface expressions of a deep-subterranean sedimentary volcanism, are widespread throughout the world. The methane and sulfur cycles are recognized as the most important biogeochemical cycles in these environments. Only few anaerobic bacterial strains were recovered from TMVs.

Direct Measurement of the Four-Phase Equilibrium Coexistence Vapor-Aqueous Solution-Ice-Gas Hydrate in Water-Carbon Dioxide System.

Precise data on the non-variant equilibrium of the four phases (vapor-aqueous solution-ice-gas hydrate) in - coordinates are highly desired for developing accurate thermodynamic models and can be used as reference points (similar to the triple point of water). Using the two-component hydrate-forming system CO-HO, we have proposed and validated a new express procedure for determining the temperature and pressure of the lower quadruple point Q. The essence of the method is the direct measurement of these parameters after the successive formation of the gas hydrate and ice phases in the initial two-phase gas-water solution system under intense agitation of the fluids.

Fontisphaera persica gen. nov., sp. nov., a thermophilic hydrolytic bacterium from a hot spring of Baikal lake region, and proposal of Fontisphaeraceae fam. nov., and Limisphaeraceae fam. nov. within the Limisphaerales ord. nov. (Verrucomicrobiota).

A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 , was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30-57 °C and within a pH range of 5.

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium.

One of the important current issues of bioenergetics is the establishment of the thermodynamic limits of life. There is still no final understanding of what is the minimum value of the energy yield of a reaction that is sufficient to be used by an organism (the so-called "biological quantum of energy"). A reasonable model for determination of the minimal energy yield would be microorganisms capable of living on low-energy substrates, such as acetogenic prokaryotes.

Laser Derived Electron Transport Layers with Embedded p-n Heterointerfaces Enabling Planar Perovskite Solar Cells with Efficiency over 25.

Electron transport layers (ETLs) with pronounced electron conducting capability are essential for high performance planar perovskite photovoltaics, with the great challenge being that the most widely used metal oxide ETLs unfortunately have intrinsically low carrier mobility. Herein is demonstrated that by simply addressing the carrier loss at particle boundaries of TiO ETLs, through embedding in ETL p-n heterointerfaces, the electron mobility of the ETLs can be boosted by three orders of magnitude. Such embedding is encouragingly favorable for both inhibiting the formation of rutile phase TiO in ETL, and initiating the growth of high-quality perovskite films with less defect states.

Fontivita pretiosa gen. nov., sp. nov., a thermophilic planctomycete of the order Tepidisphaerales from a hot spring of Baikal lake region.

A novel facultatively anaerobic moderately thermophilic bacterium, strain B-254, was isolated from a terrestrial hot spring near the town of Goryachinsk in the Baikal lake region (Russian Federation). Motile spherical cells of the strain were present as single cocci, in pairs, or aggregates. The cells had a Gram negative cell wall and reproduced by binary fission.

Epinephrine extensively changes the biofilm matrix composition in C01 isolated from human skin.

The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal C01 and its biofilms, and also the matrix changes during the biofilm growth. Epinephrine in concentration 4.

Natural Nanoclay-Based Silver-Phosphomolybdic Acid Composite with a Dual Antimicrobial Effect.

The problem of microbial growth on various surfaces has increased concern in society in the context of antibiotic misuse and the spreading of hospital infections. Thus, the development of new, antibiotic-free antibacterial strategies is required to combat bacteria resistant to usual antibiotic treatments. This work reports a new method for producing an antibiotic-free antibacterial halloysite-based nanocomposite with silver nanoparticles and phosphomolybdic acid as biocides, which can be used as components of smart antimicrobial coatings.

Activating a Semiconductor-Liquid Junction via Laser-Derived Dual Interfacial Layers for Boosted Photoelectrochemical Water Splitting.

The semiconductor-liquid junction (SCLJ), the dominant place in photoelectrochemical (PEC) catalysis, determines the interfacial activity and stability of photoelectrodes, whcih directly affects the viability of PEC hydrogen generation. Though efforts dedicated in past decades, a challenge remains regarding creating a synchronously active and stable SCLJ, owing to the technical hurdles of simultaneously overlaying the two advantages. The present work demonstrates that creating an SCLJ with a unique configuration of the dual interfacial layers can yield BiVO photoanodes with synchronously boosted photoelectrochemical activity and operational stability, with values located at the top in the records of such photoelectrodes.

sp. nov., an anaerobic bacterium isolated from a coastal lake.

A novel anaerobic chemoorganotrophic, facultatively alkaliphilic bacterium (strain M17 DMB) was isolated from a coastal lake (Golubitsckoe, Taman Peninsula, Russia). Cells were motile rods, 1.6-2.

Dataset for the interfacial tension and phase properties of the ternary system water - 2-butoxyethanol - toluene.

Two-phase samples containing water, 2-butoxyethanol, and toluene in the different mass ratios were gravimetrically prepared in the jacketed cells at =293.15 K and =0.100 MPa and equilibrated for 24 h.

Single crystals of undoped LiWO and LiWMoO: formation enthalpies, heat capacity in the temperature range 320-997 K.

The LiWO single crystal was first grown by applying the Czochralski technique with weight control and low-temperature-gradients. A single crystal of LiWO is one of the perspective materials for researching rare events. The heat capacity for a LiWMoO single crystal has been determined by DSC calorimetry in the temperature range 320-997 K for the first time.

Novel hyperthermophilic crenarchaeon Infirmifilum lucidum gen. nov. sp. nov., reclassification of Thermofilum uzonense as Infirmifilum uzonense comb. nov. and assignment of the family Thermofilaceae to the order Thermofilales ord. nov.

A novel hyperthermophilic crenarchaeon, strain 3507LT, was isolated from a terrestrial hot spring near Tinguiririca volcano, Chile. Cells were non-motile thin, slightly curved filamentous rods. It grew at 73-93 °C and pH range of 5 to 7.

Detection of bacterial colonization by the spectral changes of surface-enhanced Raman reporters.

In times of widespread multiple antibiotic resistance, the bacterial colonization of crucial medical surfaces should be detected as fast as possible. In this work, we present the non-destructive SERS method for the detection of bacterial colonization. SERS is an excellent tool for the monitoring of suitable substances in low concentrations.

Thermogemmata fonticola gen. nov., sp. nov., the first thermophilic planctomycete of the order Gemmatales from a Kamchatka hot spring.

A novel aerobic moderately thermophilic bacterium, designated strain 2918, was isolated from a terrestrial hot spring of Kamchatka, Russian Federation. Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by budding. The strain grew at 25-60°C and within a pH range of 5.

Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia.

A novel anaerobic moderately thermophilic bacterium, designated strain 38H-str, was isolated from a 12m deep hot spring of the Kunashir Island shore. Gram-negative cells were non-spore-forming, motile, straight or curved filamentous rods, occasionally forming loops and knots. The strain grew at 20-65°C and pH range of 4.

Highly Stable Energy Capsules with Nano-SiO Pickering Shell for Thermal Energy Storage and Release.

Phase change materials (PCMs) store latent heat energy as they melt and release it upon freezing. However, they suffer from chemical instability and poor thermal conductivity, which can be improved by encapsulation. Here, we encapsulated a salt hydrate PCM (Mg(NO)·6HO) within all-silica nanocapsules using a Pickering emulsion template.

sp. nov., a bacterium isolated from Chukotka hot spring, Arctic region, Russia.

A moderately thermophilic, neutrophilic, aerobic, Gram-negative bacterium, strain 3729k, was isolated from a thermal spring of the Chukotka Peninsula, Arctic region, Russia. It grew chemoorganoheterotrophically, utilizing proteinaceous substrates, including highly rigid keratins as well as various polysaccharides (glucomannan, locust bean gum, gum guar and xanthan gum). The major fatty acids of strain 3729k were iso-C (60.

Analysis of Natural Gas Using a Portable Hollow-Core Photonic Crystal Coupled Raman Spectrometer.

The low accessibility of natural gas fields and transporting pipelines requires portable online analyzers of the composition of natural gas, ensuring nearly chromatographic precision and capable of in situ analysis of a wide range of gases, including infrared-inactive ones (hydrogen, oxygen, nitrogen, chlorine). We have developed an express method of gas analysis meeting all the requirements for analysis of natural gas and its derivative mixtures using a portable 532 nm Raman spectrometer rigidly connected to a hollow-core crystal photonic fiber.