Biocatalytic Performance of -Glucosidase Immobilized on 3D-Printed Single- and Multi-Channel Polylactic Acid Microreactors.

Microfluidic devices have attracted much attention in the current day owing to the unique advantages they provide. However, their application for industrial use is limited due to manufacturing limitations and high cost. Moreover, the scaling-up process of the microreactor has proven to be difficult.

3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization.

3D printing is lately utilized in biological sciences under the scope to develop customized scaffolds that will host biomolecules, either whole cells or parts of them, like enzymes. In the present work, we present a protocol to modify the surface of 3D printed polylactic acid (PLA) well-plates with the aim to co-immobilize multiple enzymes that will perform cascade reactions. Detailed steps to design and print the final models are described.