Functions of corazonin and histamine in light entrainment of the circadian pacemaker in the Madeira cockroach, Rhyparobia maderae.

The circadian pacemaker of the Madeira cockroach, Rhyparobia (Leucophaea) maderae, is located in the accessory medulla (AME). Ipsi- and contralateral histaminergic compound eyes are required for photic entrainment. Light pulses delay locomotor activity rhythm during the early night and advance it during the late night.

Peptidergic circadian clock circuits in the Madeira cockroach.

Circadian clocks control physiology and behavior of organisms in synchrony with external light dark cycles in changing photoperiods. The Madeira cockroach Rhyparobia maderae was the first model organism in which an endogenous circadian clock in the brain was identified. About 240 neurons constitute the cockroach circadian pacemaker network in the accessory medulla.

The neuropeptide SIFamide in the brain of three cockroach species.

The sequence as well as the distribution pattern of SIFamide in the brain of different insects is highly conserved. As a general rule, at least four prominent SIFamide-immunoreactive somata occur in the pars intercerebralis. They arborize throughout the brain and the ventral nerve cord.

Generation and flow cytometric quality control of clinical-scale TCRαβ/CD19-depleted grafts.

Background: The depletion of TCRαβ T cells and CD19 B cells is a graft purification method for haploidentical stem cell transplantation (HSCT) retaining stem cells, NK cells and TCRγδ T cells. To avoid treatment-related occurrence of severe GvHD a precise quantification of residual TCRαβ T cells in the graft is of essential importance.

Methods: Nine stem cell grafts were purified immunomagnetically on a CliniMACS device and flow cytometric quality control (QC) was performed before and after TCRαβ/CD19-depletion.

Bimodal oscillations of cyclic nucleotide concentrations in the circadian system of the Madeira cockroach Rhyparobia maderae.

Pigment-dispersing factor (PDF) is the most important coupling factor of the circadian system in insects, comparable to its functional ortholog vasoactive intestinal polypeptide of the mammalian circadian clock. In Drosophila melanogaster, PDF signals via activation of adenylyl cyclases, controlling circadian locomotor activity rhythms at dusk and dawn. In addition, PDF mediates circadian rhythms of the visual system and is involved in entrainment to different photoperiods.