Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae infection.

Plant response to pathogen infection varies within a leaf, yet this heterogeneity is not well resolved. We expose Arabidopsis to Pseudomonas syringae or mock treatment and profile >11,000 individual cells using single-cell RNA sequencing. Integrative analysis of cell populations from both treatments identifies distinct pathogen-responsive cell clusters exhibiting transcriptional responses ranging from immunity to susceptibility.

A Genetic Toolkit for Investigating Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid.

The development of knockout mutants and expression variants are critical for understanding genotype-phenotype relationships. However, advances in these techniques in gram-positive actinobacteria have stagnated over the last decade. Actinobacteria in the genus are composed of diverse crop pathogens that cause a variety of wilt and cankering diseases.

Long-Term Social Outcomes of Hyperfractionated Radiation on Childhood ALL Survivors.

Background/objectives: Childhood cranial radiation has irreversible neurocognitive effects. Hyperfractionated radiation therapy (HFX) for acute lymphoblastic leukemia (ALL) was randomized against conventionally fractionated radiation therapy (CFX) in the DFCI 87-01/91-01 trials attempting to minimize these effects. When neurocognitive testing 8-year posttreatment demonstrated no difference, this strategy was abandoned.