The Influence of Commonly-Encountered Participant Behaviors on Cardiovascular Indices during Testing.

Background: During laboratory testing, participants rest quietly in a supine posture with little movement. However, it is rather common for participants to display various behaviors. The extent to which these common encounters influence cardiovascular measures is unknown.

Single Nucleotide Polymorphisms Associated with Prostate Cancer Progression: A Systematic Review.

Background: New biomarkers of progression in patients with prostate cancer (PCa) are needed to improve their classification and clinical management. This systematic review investigated the relationship between single nucleotide polymorphisms (SNPs) and PCa progression.

Methods: A keyword search was performed in Pubmed, EMBASE, Scopus, Web of Science, and Cochrane for publications between 2007 and 2022.

Valorization of lead-zinc mine tailing waste through geopolymerization: Synthesis, mechanical, and microstructural properties.

Lead-zinc mine tailing waste can have significant environmental impacts due to its potential for releasing toxic elements into the surroundings and contaminating local soil and water. This paper focuses on the valorization of lead-zinc mine tailing waste through geopolymerization, a sustainable process that can transform waste into useful building materials. Geopolymer matrixes with various mixtures of mine tailing (0-100 wt%), fly ash (0-100 wt%), and flue gas desulfurization (FGD) gypsum (0, 5, and 10 wt%) were synthesized using different activators such as sodium hydroxide (NaOH, 5, 10 M) and sodium silicate (waterglass, 0, 12.

Consensus on the therapeutic management of atopic dermatitis ‒ Brazilian Society of Dermatology: an update on phototherapy and systemic therapy using e-Delphi technique.

This publication is an update of the "Consensus on the therapeutic management of atopic dermatitis - Brazilian Society of Dermatology" published in 2019, considering the novel, targeted-oriented systemic therapies for atopic dermatitis. The initial recommendations of the current consensus for systemic treatment of patients with atopic dermatitis were based on a recent review of scientific published data and a consensus was reached after voting. The Brazilian Society of Dermatology invited 31 experts from all regions of Brazil and 2 international experts on atopic dermatitis who fully contributed to the process.

Ultrasound-assisted enzymatic indirect determination of total 3-monochloropropane-1,2-diol esters in canned fish oil fraction.

A novel, fast, and cost-effective indirect enzymatic method was successfully developed to assess the total 3-monochloropropane-1,2-diol (3-MCPD) in canned food's oil fraction by the action of Burkholderia cepacia lipase. The total 3-MCPD were derivatized with n-Heptafluorobutyrylimidazole (HFBI) for GC-MS analysis during dispersive liquid-liquid microextraction (DLLME). An asymmetrical 21//8 screening design was used to study the influence of critical factors on the method's effectiveness.

Arsenic(V) immobilization in fly ash and mine tailing-based geopolymers: Performance and mechanism insight.

Global mining activities produce thousands of millions of toxic-bearing mine tailing (MT) wastes each year. Storage of the mine tailings not only encroaches upon large areas of cropland but also arouses additional ecological and environmental risks. Herein we demonstrate that geopolymerization of a mixture of the toxic-bearing mine tailings and the coal fly ash (FA) can effectively immobilize exogenous arsenic (As) species in addition to inherent As from the raw materials.

International eDelphi Study to Reach Consensus on the Methotrexate Dosing Regimen in Patients With Psoriasis.

Importance: A clear dosing regimen for methotrexate in psoriasis is lacking, and this might lead to a suboptimal treatment. Because methotrexate is affordable and globally available, a uniform dosing regimen could potentially optimize the treatment of patients with psoriasis worldwide.

Objective: To reach international consensus among psoriasis experts on a uniform dosing regimen for treatment with methotrexate in adult and pediatric patients with psoriasis and identify potential future research topics.

Opioid overdoses: Emerging clinical challenges.

Objective: Analysis of a large consecutive case series of cases brought to an Emergency Department (ED).

Design: Retrospective chart review.

Setting: Emergency Department in Broward County, Florida.

Trends in extreme rainfall and hydrogeometeorological disasters in the Metropolitan Area of São Paulo: a review.

Our paper reviews recent progress in the study and understanding of observed trends in extreme rainfall events in the Metropolitan Area of São Paulo (MASP). These are discussed in relation to hydrometeorological hazards that trigger natural disasters, such as flash floods, landslides, and droughts, that affect the population and local economies. A review of the most updated literature on rainfall and extremes in the MASP shows a significant increase in the total volume of rainy-season rainfall during the last seven decades.

Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm (J.E. Smith, 1797) (Lepidoptera: Noctuidae).

The ability of the isolate VG008 of S. frugiperda granulovirus (SpfrGV) to enhance the infectivity of the isolate SfCOL of S. frugiperda multiple nucleopolyhedrovirus (SpfrMNPV) was evaluated on larvae.

Rifamycin derivatives active against pathogenic rapidly-growing mycobacteria.

Infections due to rapidly growing mycobacteria (RGM), and in particular the RGM species Mycobacterium abscessus (Mab), are very difficult to treat and reports on novel therapeutic options are scarce. A hallmark of all pathogenic RGM species is their resistance to the four first-line drugs used to treat infections with Mycobacterium tuberculosis including rifampicin. This study demonstrates that modification of the rifampicin scaffold can restore rifampicin activity against the three most commonly isolated pathogenic RGM species including Mab.

Consensus on the therapeutic management of atopic dermatitis - Brazilian Society of Dermatology.

Background: Atopic dermatitis is a highly prevalent inflammatory and pruritic dermatosis with a multifactorial etiology, which includes skin barrier defects, immune dysfunction, and microbiome alterations. Atopic dermatitis is mediated by genetic, environmental, and psychological factors and requires therapeutic management that covers all the aspects of its complex pathogenesis.

Objectives: The aim of this article is to present the experience, opinions, and recommendations of Brazilian dermatology experts regarding the therapeutic management of atopic dermatitis.

Consensus on the diagnostic and therapeutic management of chronic spontaneous urticaria in adults - Brazilian Society of Dermatology.

Background: Urticarias are frequent diseases, with 15% to 20% of the population presenting at least one acute episode in their lifetime. Urticaria are classified in acute ( ≤ 6 weeks) or chronic (> 6 weeks). They may be induced or spontaneous.

Diagnostic and therapeutic guidelines for plaque psoriasis - Brazilian Society of Dermatology.

Psoriasis is a chronic inflammatory disease that affects 1.3% of the Brazilian population. The most common clinical manifestations are erythematous, scaling lesions that affect both genders and can occur on any anatomical site, preferentially involving the knees, elbows, scalp and genitals.

Consensus on the treatment of hidradenitis suppurativa - Brazilian Society of Dermatology.

Hidradenitis suppurativa is a chronic immune mediated disease of universal distribution that causes great damage to the quality of life of the affected individual, whose prevalence is estimated at 0.41% in the Brazilian population. The objective of this work was update on physiopathogenesis, diagnosis and classification of hidradenitis suppurativa and to establish therapeutic recommendations in the Brazilian reality.

Profile of dermatological consultations in Brazil (2018).

Background: Dermatological diseases are among the primary causes of the demand for basic health care. Studies on the frequency of dermatoses are important for the proper management of health planning.

Objectives: To evaluate the nosological and behavioral profiles of dermatological consultations in Brazil.

Identifying Potential Sporozoite Stage Vaccine Candidates: An Analysis of Genetic Diversity and Natural Selection.

Parasite antigen genetic diversity represents a great obstacle when designing a vaccine against malaria caused by . Selecting vaccine candidate antigens has been focused on those fulfilling a role in invasion and which are conserved, thus avoiding specific-allele immune responses. Most antigens described to date belong to the blood stage, thereby blocking parasite development within red blood cells, whilst studying antigens from other stages has been quite restricted.

A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA.

Background: Neospora caninum is a cyst-forming, coccidian parasite which is known to cause neurological disorders in dogs and abortion and neonatal mortality in cows and other livestock. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay based on the Neospora caninum Nc-5 gene and compares its efficacy for detecting DNA to that of a semi-nested PCR test.

Results: Six primers were designed based on the Nc-5 repeat region of N.

Uniform multidrug therapy for leprosy patients in Brazil (U-MDT/CT-BR): Results of an open label, randomized and controlled clinical trial, among multibacillary patients.

Background: Leprosy control is based on early diagnosis and multidrug therapy. For treatment purposes, leprosy patients can be classified as paucibacillary (PB) or multibacillary (MB), according to the number of skin lesions. Studies regarding a uniform treatment regimen (U-MDT) for all leprosy patients have been encouraged by the WHO, rendering disease classification unnecessary.

Acute and long-term effects of intracerebroventricular administration of α-ketoisocaproic acid on oxidative stress parameters and cognitive and noncognitive behaviors.

Maple Syrup Urine Disease (MSUD) is biochemically characterized by elevated levels of leucine, isoleucine and valine, as well as their corresponding transaminated branched-chain α-keto acids in tissue and biological fluids. Neurological symptoms and cerebral abnormalities, whose mechanisms are still unknown, are typical of this metabolic disorder. In the present study, we evaluated the early effects (1 h after injection) and long-term effects (15 days after injection) of a single intracerebroventricular administration of α-ketoisocaproic acid (KIC) on oxidative stress parameters and cognitive and noncognitive behaviors.

Improving myoblast differentiation on electrospun poly(ε-caprolactone) scaffolds.

Polymer scaffolds are used as an alternative to support tissue regeneration when it does not occur on its own. Cell response on polymer scaffolds is determined by factors such as polymer composition, topology, and the presence of other molecules. We evaluated the cellular response of murine skeletal muscle myoblasts on aligned or unaligned fibers obtained by electrospinning poly(ε-caprolactone) (PCL), and blends with poly(lactic-co-glycolic acid) (PLGA) or decorin, a proteoglycan known to regulate myogenesis.

pELMO, an optimised in-house cloning vector.

DNA cloning is an essential tool regarding DNA recombinant technology as it allows the replication of foreign DNA fragments within a cell. pELMO was here constructed as an in-house cloning vector for rapid and low-cost PCR product propagation; it is an optimally designed vector containing the ccdB killer gene from the pDONR 221 plasmid, cloned into the pUC18 vector's multiple cloning site (Thermo Scientific). The ccdB killer gene has a cleavage site (CCC/GGG) for the SmaI restriction enzyme which is used for vector linearisation and cloning blunt-ended products.

Host Cell Copper Transporters CTR1 and ATP7A are important for Influenza A virus replication.

Background: The essential role of copper in eukaryotic cellular physiology is known, but has not been recognized as important in the context of influenza A virus infection. In this study, we investigated the effect of cellular copper on influenza A virus replication.

Methods: Influenza A/WSN/33 (H1N1) virus growth and macromolecule syntheses were assessed in cultured human lung cells (A549) where the copper concentration of the growth medium was modified, or expression of host genes involved in copper homeostasis was targeted by RNA interference.

Chitosan/poly-octanoic acid 2-thiophen-3-yl-ethyl ester blends as a scaffold to maintain myoblasts regeneration potential in vitro.

Satellite cells are a small cell population that function as muscle-specific adult stem cells. When muscle damage occurs, these cells are able to activate, proliferate, and ultimately fuse with each other in order to form new myofibers or fuse with existing ones. For tissue engineering applications, obtaining a sufficient number of myoblasts prior transplantation that maintains their regenerative capacity is critical.