Publications by authors named "Andrea Pelikan-Conchaudron"
IQGAP1 is a large modular protein that displays multiple partnership and is thought to act as a scaffold in coupling cell signaling to the actin and microtubule cytoskeletons in cell migration, adhesion, and cytokinesis. However the molecular mechanisms underlying the activities of IQGAP1 are poorly understood in part because of its large size, poor solubility and lack of functional assays to challenge biochemical properties in various contexts. We have purified bacterially expressed recombinant human IQGAP1.
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- The hydrolysis of ATP during actin polymerization affects filament stability and assembly dynamics, influencing how regulatory proteins bind to actin.
- Labeling actin with tetramethylrhodamine (TMR) prevents polymerization and allows stabilized crystal structures of ADP-actin and its complexes with formin proteins to be examined.
- TMR labeling alters actin's assembly properties and interactions with regulatory proteins, suggesting that it restricts the structural flexibility necessary for effective binding and function in both ATP and ADP states.