Publications by authors named "Andrea Kinnear"
Mouse is the mammalian model of choice to study human health and disease due to its size, ease of breeding and the natural occurrence of conditions mimicking human pathology. Here we design and validate multiple reaction monitoring mass spectrometry (MRM-MS) assays for quantitation of 2118 unique proteins in 20 murine tissues and organs. We provide open access to technical aspects of these assays to enable their implementation in other laboratories, and demonstrate their suitability for proteomic profiling in mice by measuring normal protein abundances in tissues from three mouse strains: C57BL/6NCrl, NOD/SCID, and BALB/cAnNCrl.
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- Traditional studies on Mycoplasma bovis and antimicrobial resistance (AMR) focused on specific genetic locations, but this research used a genome-wide association study (GWAS) approach on 194 M. bovis genomes instead.
- Two models (fixed effects linear model and linear mixed model) were used to find links between genetic variants and resistance to various antibiotics, identifying both known and new nucleotide variants.
- The study highlighted that resistance to antibiotics was associated with several genes, including those related to ribosomal proteins and ABC transporters, indicating a complex, multi-gene involvement in AMR in M. bovis.
A comparative study of microbiota of the respiratory tract and joints of bovine respiratory disease (BRD) cattle mortalities was undertaken. Nasopharynx, trachea, lung and joint samples were collected from 32 cattle that died of BRD, "cases", and 8 that died of other causes, "controls". Bacterial diversity was lower ( < 0.
View Article and Find Full Text PDFMycoplasma bovis is a significant pathogen of feedlot cattle, responsible for chronic pneumonia and polyarthritis syndrome (CPPS). M. bovis isolates (= 129) were used to compare four methods of phylogenetic analysis and to determine if the isolates' genotypes were associated with phenotypes.
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- - The study investigates the role of mycoplasmosis in causing bovine respiratory disease and chronic pneumonia in feedlot cattle, focusing on antimicrobial resistance to commonly used macrolide antibiotics.
- - Whole genome sequencing was performed on 126 bacterial isolates from feedlot cattle, with antimicrobial susceptibility testing revealing that specific mutations in the 23S rRNA gene were linked to resistance against macrolides like tilmicosin and tildipirosin.
- - Despite varying mutations, over 99% of isolates showed resistance to tildipirosin and tilmicosin, while isolates with certain mutations remained susceptible only to tulathromycin.
is particularly adept at evading the immune system, resulting in chronic infections of the lungs and joints of feedlot cattle. The chronicity of the lesions results in prolonged antimicrobial therapy, possibly exacerbating antimicrobial resistance. This cross-sectional study generated in vitro antimicrobial susceptibility testing (AST) data on 211 isolates recovered from 159 healthy, diseased, and dead cattle, spanning the period of 2006-2018.
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