Publications by authors named "Andrea Caetano Da Silva"

Bovine neosporosis is a parasitic disease with worldwide distribution that causes important economic losses. Because of the limited information on the occurrence of infection in Colombia, this study aimed to determine the seroprevalence and identify the risk factors associated with this infection in cattle in Antioquia, which is the largest milk-producing state in the country. We collected 1,038 blood samples from Holstein, Jersey and crossbred cows from 31 farms.

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Article Synopsis
  • The study applied Neospora caninum microsatellite markers to analyze clinical samples from infected zebuine foetuses in Goiás, Brazil, focusing on DNA from 15 brains and four hearts.
  • Using fluorescently labeled DNA fragment analysis and sequencing, researchers obtained distinct allele profiles from the samples, discovering three different genotypes unique to the region and a new allele.
  • The findings indicate that there are genetic differences in N. caninum strains affecting foetal transmission in South America, marking a significant advancement in understanding these infections.
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Neosporosis is considered to be one of the main causes of abortions in bovines. In this study we evaluated the congenital transmission and occurrence of abortions by Neospora caninum in an embryo transfer center in Neropolis, Goiás. Serological samples from 101 recipients, 61 donors, and 90 calves were analyzed.

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This study was developed to evaluate the repellent activity of N,N-diethyl-3-methylbenzamide (DEET) against Amblyomma cajennense nymphs. Two repellent bioassays were compared and the effective concentration and repellent time were calculated. The fingertip test was accomplished to evaluate in vivo four concentrations of the compound (0.

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Tritrichomonas foetus is a pathogenic protozoan that causes a venereal disease in cattle known as bovine genital tricomonosis. In spite of the efficacy to recognize the target genomic DNA, the protocols so far developed for the diagnosis of this organism by PCR promote some inespecific amplifications or they are unable to discriminate T. foetus against other species within the genus.

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  • * Out of 20 seropositive bulls, 40% had at least one semen straw testing positive for N. caninum DNA, but the overall detection rate was low at 7.8%.
  • * The findings suggest that while N. caninum was occasionally found in bull semen, the low frequency and quantity of the parasite reduce the risk of transmission through artificial insemination (AI).
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  • Neospora caninum is primarily transmitted to cattle through transplacental infection, but there's a possibility of postnatal transmission via sporozoite-containing oocysts from dogs.
  • A study tested for N. caninum DNA in semen from five Holstein-Friesian bulls infected with neosporosis, using nested-PCR and other methods to confirm infection.
  • N. caninum DNA was found in small amounts in non-extended fresh semen and frozen semen straws of infected bulls, while control bulls showed no presence of the parasite, indicating more research is needed to explore venereal transmission routes.
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