Neo1 and phosphatidylethanolamine contribute to vacuole membrane fusion in .

is an essential gene in budding yeast and belongs to a highly conserved subfamily of P-type ATPase genes that encode phospholipid flippases. Inactivation of temperature sensitive alleles produces pleiomorphic defects in the secretory and endocytic pathways, including fragmented vacuoles. A screen for multicopy suppressors of growth defects yielded , which encodes a Rab7 homolog involved in SNARE-dependent vacuolar fusion.