Purification and partial characterization of a novel lectin from Dioclea lasiocarpa Mart seeds with vasodilator effects.

A lectin from seeds of Dioclea lasiocarpa (DLL) was purified in a single step by affinity chromatography in a Sephadex G-50 column. DLL haemagglutinated rabbit erythrocytes showing stability even after 1 h of exposure to a different pH values (optimal between pH 6.0 and 8.

Production of Abrus pulchellus ribosome-inactivating protein from seeds callus culture.

Ribosome inactivating proteins (RIPs) were isolated from callus culture that were established from seed explants of Abrus pulchellus. Cotyledon segments of immature seeds were inoculated in basal medium MS supplemented with different concentrations of auxin (2,4-D), citokinin (kinetin and BA) and sucrose in order to determine the best callus induction. A.