Accumulation of each element in the lysosome of tumor and liver cells as systematized according to its location in the periodic table.

Hard and borderline acids which were formed by losing electrons from the d-shell accumulated extensively in the lysosomes of tumor and liver with time after administration of these ions. They were bound in these tissues to acid mucopolysaccharides, the mol. wt of which exceed 40,000 Da.

Mechanism of gallium 67 accumulation in inflammatory tissue.

The present study was undertaken to elucidate the accumulation mechanism of gallium 67 in inflammatory tissue. 67Ga accumulation in inflammatory tissue was observed by macro- and microautoradiogram. Permeability indices were calculated for serum albumin from blood vessels into inflammatory and normal tissue.

Importance of DQB as an indicator in living-related kidney transplant.

Southern blot hybridization was performed in 16 pairs of living-related kidney transplant patients and donors, using DNA samples extracted from peripheral blood lymphocytes. The number of HLA-DNA-mismatched bands was used as an indicator for graft survival. The total number of DNA-mismatched bands seemed to be a valuable parameter.

Tumor uptake and biodistribution of various radiolabels.

42K, 86Rb, 134Cs and 201Tl accumulated in viable tumor tissue and not in necrotic tumor tissue. 22Na, however, accumulated in necrotic tumor tissue. These elements existed almost as free ions in the tissues.

Influence of vitamin B2 on formation of chromium(V), alkali-labile sites, and lethality of sodium chromate(VI) in Chinese hamster V-79 cells.

The effect of vitamin B2 on the cellular reduction and cytotoxicity of chromate(VI) was studied using Chinese hamster V-79 cells. Electron spin resonance studies showed that incubation of cells with Na2CrO4 resulted in the formation of both chromium(V) and chromium(III) complex and that cellular pretreatment with riboflavin (Vitamin B2) for 24 h prior to exposure increased the level of chromium(V) complex, but the level of chromium(III) remained unchanged. Analysis of flavin derivatives revealed that pretreatment with vitamin B2 increased free riboflavin without altering flavin adenine dinucleotide and flavin mononucleotide.

Effects of dietary protein intake on renal function in humans.

The effects of an acute protein load on renal hemodynamic responses and plasma glucagon levels were investigated in 31 patients with biopsy proven chronic glomerulonephritis (24 cases) or chronic renal failure (6 cases). After baseline clearance measurements, the subjects ingested a high protein meal consisting of 1.2 to 1.

[Fate of the false lumen after surgery and assessment of operative procedure for DeBakey IIIb dissecting aortic aneurysm].

Eighteen patients with DAA IIIb were divided into two groups. Seven patients with major abdominal arteries originated from true lumen (group I) and 11 ones with renal artery or arteries from false lumen (group II). group I: In early postoperative phase (25-55 days), the descending aortic false lumen had been thrombo-occluded in all patients and the upper abdominal aortic false lumen had been still enhanced in three.

HLA-D typing of heterozygotes using restriction fragment length polymorphism in the HLA-DQ gene region on the basis of standard band patterns derived from HLA homozygotes.

Genomic DNAs from 37 Epstein-Barr virus-transformed HLA-D homozygous cell lines with different Dw specificities, Dw1-Dw23, and DKT2 were digested with four different restriction endonucleases (EcoRI, PstI, TaqI, and MspI) and hybridized to DQ alpha and DQ beta cDNA probes. Polymorphic patterns of multiple fragments correlating with Dw, DQ, and DR haplotypes were detected, and the restriction fragment length polymorphism standard pattern specific for each Dw, DQ, and DR specificity could be defined. The polymorphic fragment patterns of HLA-D heterozygotes were predicted simply by the summation of two standard patterns of HLA-D homozygotes and utilized to identify HLA-D specificities of 25 normal individuals, who are HLA heterozygotes in most cases.

[Comparison between pre- and post-operative renal function and fate of the false lumen after surgical treatment of dissecting aortic aneurysms].

Twenty-seven patients, whose renal artery (or arteries) originated from false lumen, were studied dividing into two groups based on pre-operative renal state. Group I, without morphological and functional disorder, did not show any change pre- and post-operatively. Post-operative angiography indicated that pre-operative re-entry played as a entry to perfuse the false lumen originated renal artery (or arteries).

Effect of vitamin E on survival, glutathione reductase and formation of chromium (V) in Chinese hamster V-79 cells treated with sodium chromate (VI).

The effect of vitamin E on cytotoxicity induced by Na2CrO4 was evaluated by colony-forming assay using Chinese hamster V-79 cells. Pre-treatment with alpha-tocopherol succinate (vitamin E) for 24 h prior to exposure to Na2CrO4 resulted in a marked decrease in the cytotoxicity caused by this compound. The reduction of chromate-induced cytotoxicity was observed at all concentrations of Na2CrO4 (5-15 microM), and the protective effect increased with higher concentrations of vitamin E (5-25 microM).

Vitamin B2-enhancement of sodium chromate (VI)--Induced DNA single strand breaks: ESR study of the action of vitamin B2.

Incubation of Chinese hamster V-79 cells with Na2CrO4 plus vitamin B2 resulted in an increase of Na2CrO4-induced DNA single strand breaks. Electron spin resonance (ESR) studies showed that vitamin B2 enhanced the formation of both hydroxyl radical and tetraperoxochromate (V) during the reaction of Na2CrO4 with hydrogen peroxide. Furthermore, ESR studies demonstrated that a chromium (V) species with a g value of 1.

No difference in the nucleotide sequence of the DQ beta beta 1 domain between narcoleptic and healthy individuals with DR2,Dw2.

Narcolepsy is a sleep disorder completely associated with HLA-DR2,Dw2. We demonstrated the 100% presence of three DQ beta fragments (EcoRI 2.4-kb, BamHI 2.

Renal and hemodynamic effects of synthetic atrial natriuretic peptide in dogs with chronic renal failure.

The present experiments were performed to clarify the renal and hemodynamic effects of atrial natriuretic peptide (ANP) in dogs with chronic renal failure (CRF; produced by 5/6 nephrectomy 3-4 weeks before study). Synthetic alpha-hANP was administered intravenously (0.1 micrograms/kg body weight for 30 min) with a priming bolus injection (1.

Effects of atrial natriuretic peptide on regional renal blood flow measured by a thermal diffusion technique.

The effects of atrial natriuretic peptide (ANP) on the regional renal blood flow were examined in anesthetized dogs. A thermal diffusion technique was applied in an attempt to observe the intrarenal blood distribution. Tissue blood flow was continuously measured according to the changes of the thermal gradient produced by a semiconductor (Peltier effect).

Relation between the location of elements in the periodic table and various organ-uptake rates.

Fifty four elements and 65 radioactive compounds were examined to determine the organ uptake rates for rats 3, 24 and 48 h after i.v. injection of these compounds.

Mapping and nucleotide sequence of a new HLA class II light chain gene, DQB3.

A genomic clone specifying a new HLA class II antigen beta chain, DQB3, was isolated from a human genomic phage library using a DQB1 cDNA probe under low stringency conditions. Southern hybridization and nucleotide sequence analyses identified the beta 2 domain exon (exon 3) with several deleterious mutations and the CP-TM-CY exon [connecting peptide, transmembrane, and cytoplasmic regions, (exon 4)], but the first, second, and fifth exons encoding the 5' UT-leader, the beta 1 domain, and the 3' UT domain of normal beta chains, respectively, were entirely missing. The nucleotide sequences of these two exons were distinct from those of other class II beta chain genes, but slightly more related to the DQB1 and DQB2 genes than to other class II genes.

Determination of the lysosomal role in tumor accumulation of 67Ga by dual-tracer studies.

The lysosomal role in tumor accumulation of 67Ga was determined by dual-tracer (67Ga and 46Sc) studies. It became clear that 67Ga essentially did not accumulate in the tumor lysosome, and that the lysosome did not play a major role in tumor accumulation of 67Ga. In addition, it was revealed that tumor lysosome was hardly disrupted at all in some phases of fractionation procedures.

Analysis of gene structure and antigen determinants of DR2 antigens using DR gene transfer into mouse L cells.

Three HLA class II DR beta genes and one DR alpha gene from the DR2 haplotype were cloned in cosmid vectors. The DR beta II gene might be a pseudogene lacking the first exon that encodes the leader peptide. The DR beta I and DR beta III genes were expressed, together with the DR alpha-chain, after transfection into mouse L cells.

[Structure and function of human transplantation antigens].

Human transplantation antigens encoded in the major histocompatibility complex (MHC) region play a key role in regulating the immune responses. Here, we will describe the summary of our analyses on the structure and function of the human MHC molecules, HLA antigens as follows. 1) The genomic organization of the HLA antigen region was examined by cosmid cloning and pulsed-field gel electrophoresis technique.