Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing.

Ten microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main population were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C.