Marek's disease virus (MDV) latency-associated transcripts include at least two MDV small RNAs (MSRs) and a 10-kb RNA which map antisense to the ICP4 homolog gene and are relatively abundant in MDV-transformed lymphoblastoid cells. This report further describes the biological and structural properties of these RNAs. First, these RNAs were detected in primary lymphomas isolated from chickens infected with several oncogenic MDV strains.
View Article and Find Full Text PDFAn antisense strategy has been used to identify genes important for the maintenance of transformation of MDCC-MSB1 (MSB1) Marek's disease virus-transformed lymphoblastoid cells. Oligodeoxynucleotides antisense to the predicted translation initiation regions of ICP4 and pp38 mRNAs inhibited proliferation of MSB1 cells but not MDCC-CU91 (CU91) reticuloendotheliosis virus-transformed cells. Control oligodeoxynucleotides having the same base composition but a different sequence did not inhibit MSB1 cell proliferation.
View Article and Find Full Text PDFWe previously reported the construction of Marek's disease virus (MDV) strains having mutations in various genes that map to the unique short (US) region of the viral genome (J.L. Cantello, A.
View Article and Find Full Text PDFTo assess the effect of an antenatal nutrition programme designed specifically for the local population, questionnaires on nutrition knowledge, attitudes to healthier eating and 4 d diet diaries were completed by women attending routine antenatal clinics. Women who received the nutrition education programme were allocated into an Intervention Education Group whilst those women who did not were allocated into a Routine Education Group. The results showed that knowledge about nutrition was significantly higher in the intervention group.
View Article and Find Full Text PDFWe report the characterization of Marek's disease virus (MDV) strains having mutations in various genes that map to the unique short (US) region of the viral genome. A deletion mutant (GA delta 4.8lac) lacks 4.
View Article and Find Full Text PDFTwo small RNAs (0.9 and 0.75 kb), named Marek's disease virus (MDV) small RNAs (MSRs) and a 10-kb RNA, all of which map antisense to the MDV ICP4 homolog gene, have been readily detected in MDCC-MSB1 MDV-transformed T-lymphoblastoid cells.
View Article and Find Full Text PDFWe report the construction of a Marek's disease virus (MDV) mutant containing the lacZ gene of Escherichia coli inserted into a homologue of the US6 (glycoprotein D, gD) gene of herpes simplex virus. The mutant was constructed using the high-passage GAatt85 MDV strain as the parent virus, since that strain grows readily in chicken embryo fibroblasts using culture conditions conducive to mutant virus construction. The lacZ insertion site was positioned one third of the way into the US6 (gD) open reading frame.
View Article and Find Full Text PDFThe causes of nausea and vomiting in pregnancy are still not clearly understood, nor is there one simple, effective treatment. Nausea and vomiting do not appear to be associated with fetal morbidity, malnutrition or death. Carbohydrate-rich snacks at frequent intervals seem to provide relief.
View Article and Find Full Text PDFDietary data from 15-year-old adolescents participating in the West of Scotland Twenty-07 study were examined in order to describe current eating patterns and food habits. Comparison of the data with a 'healthy eating' index showed that less than one-third of the sample were eating a diet similar to that promoted by local health education campaigns. Multivariate analysis showed that adolescents from non-manual and wealthier families, non-smokers, and females were more likely to be classified as 'healthy eaters'.
View Article and Find Full Text PDFConcern is frequently expressed that young people are increasingly taking a 'grazing' approach to eating, rather than eating 'proper meals', and that this may have undesirable nutritional and social consequences. As part of a wide ranging study of health and well-being, 1,009 fifteen year olds in the Central Clydeside Conurbation were questioned in 1987 about their eating patterns. They reported an average of 5.
View Article and Find Full Text PDFThe Marek's disease virus (MDV) gene encoding a homologue to the ICP4 protein of herpes simplex virus has been mapped to BamHl fragment A based on the physical map of the MDV genome (Fukuchi et al., 1984). The gene lies completely within the inverted repeat flanking the unique short region of the genome.
View Article and Find Full Text PDFThe problem-based learning (PBL) curriculum, adopted by an increasing number of medical schools, requires retraining faculty for new roles as tutors with small groups of medical students. This study describes the procedures and results of four PBL training workshops (1989-1991) given at the University of Hawaii John A. Burns School of Medicine.
View Article and Find Full Text PDFA radioimmunoassay was developed allowing measurement of the cytotoxic cationic ECP. The assay, which has a total incubation time of 3.5 hr, is a double antibody assay with radiolabelled ECP, covering the concentration range of 2-200 micrograms/l.
View Article and Find Full Text PDFWe have isolated a stable, recombinant Marek's disease virus (MDV) containing the lacZ gene of Escherichia coli inserted into the unique short region of the genome. The nucleotide sequence of the insertion site indicates that it lies within a sequence homologous to the US2 gene of herpes simplex virus. Stable insertion of the lacZ gene into the MDV US2 gene indicates that the site is nonessential for MDV growth in cell culture.
View Article and Find Full Text PDFIn order to assess the effect of dietary advice for pregnant diabetic women, the habitual dietary intake of 8 pregnant diabetic women was assessed by 7-day weighed food records. These results were compared with records from 8 non-diabetic women, matched for age and gestation. Despite intense personalized dietetic advice to the diabetic women, there were no statistically significant differences in macro-nutrient intake between the two groups.
View Article and Find Full Text PDFMedical records were reviewed of all 263 Type 2 diabetic patients from the Aberdeen diabetic clinic who were known to have died in 1985 or 1986. Mean age was 65 years (interquartile range 57-75 years) at diagnosis and 72 (66-80) years for men, 75 (72-83) years for women, at death. Life expectancy at age 65 was 35% less than published figures for the general population.
View Article and Find Full Text PDFA low-cost locally based nutrition education programme was designed around the employment of a part-time dietitian with the aim of improving the nutritional intakes of Grampian Health Board staff. Its effectiveness was evaluated by assessment of habitual dietary intakes of staff, using a previously validated questionnaire administered to random samples before and after the intervention programme. The results indicated significant changes towards lower fat and higher carbohydrate intakes (both P less than 0.
View Article and Find Full Text PDFThis paper describes the first steps taken in Grampian Health Board to change hospital staff menus in order to provide meals higher in starchy carbohydrate and dietary fibre but lower in fat and sugar, as recommended in several recent reports. These changes are discussed with reference to the alterations made by the catering department. A nutrition education programme was used to promote these changes and the methods used for this are also described.
View Article and Find Full Text PDFBr J Obstet Gynaecol
March 1986
Using a 7-day weighed-intake method, dietary factors implicated in the aetiology of constipation were examined by comparison between the nutrient intakes of nine women with constipation in the third trimester of pregnancy who had not altered their diets, and a matched group of nine women who had not suffered constipation at any time during pregnancy. No significant differences were found for intakes of major nutrients, including dietary fibre, between the constipated and non-constipated groups, indicating that inappropriate diets do not appear to be the primary cause of constipation during pregnancy. The nutrient intakes of 40 women with constipation in the third trimester of pregnancy were examined to assess dietary changes made in attempts to treat constipation.
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