We compared the response of articular cartilage subjected to muscle stretching at different frequencies after joint immobilization. Wistar rats with immobilized left hind limbs were classified into the following groups: immobilization, immobilization followed by muscle stretching applied daily (group IS7) or three times a week (IS3), muscle stretching applied daily (S7) or three times a week (S3), and a control group (C) that underwent no intervention. We then evaluated the cartilage for cellularity, loss of proteoglycans, collagen density, and immunostaining of fibronectin and chondroitin 4-sulfate.
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