Biosynthesis of magnetite and cobalt ferrite nanoparticles using extracts of "hairy" roots: preparation, characterization, estimation for environmental remediation and biological application.

The "green" synthesis of magnetite and cobalt ferrite nanoparticles (FeO-NPs and CoFeO-NPs) using extracts of L "hairy" roots was proposed. In particular, the effect and role of important variables in the 'green' synthesis process, including the metal-salt ratio, various counter ions in the reaction mixture, concentration of total flavonoids and reducing power of the extract, were evaluated. The morphology and size distribution of the magnetic nanoparticles (MNPs) depended on the metal oxidation state and ratio of Fe(iii) : Fe(ii) in the initial reaction mixture.

'Hairy' root extracts as source for 'green' synthesis of silver nanoparticles and medical applications.

The research was focused on the synthesis of silver nanoparticles (AgNPs) using extracts from the "hairy" root cultures of Ledeb. and L. The effect of operational parameters such as type of solvent, temperature of extraction, flavonoids concentration, and reducing power of the wormwood "hairy" root extracts on the particle size and yield of the resultant nanoparticles is reported for the first time.

Agrobacterium rhizogenes-mediated transformation enhances the antioxidant potential of Artemisia tilesii Ledeb.

Plants belonging to the genus Artemisia L. have been used for medicinal purposes since ancient times. These aromatic plants produce and accumulate a wide range of potent secondary metabolites, many of which have shown antioxidant, antiparasitic, antimicrobial, anti-inflammatory, and even anticancer activities.

Flavonoid content and antioxidant activity of Artemisia vulgaris L. "hairy" roots.

We investigated the effect of Agrobacterium rhizogenes-mediated transformation on antioxidant activity of Artemisia vulgaris "hairy" roots. It appeared that transformation may increase flavonoid content as well as DPPH-scavenging activity and ability to reduce Fe as compared to the non-transformed plants. Some "hairy" roots accumulated flavonoids up to 73.

Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts.

The plastid genome of angiosperms represents an attractive target for genetic manipulations. However plastid transformation of higher plants, especially of agriculturally valuable crops is an extremely difficult problem. Transformation protocols developed for tobacco 15 years ago failed to produce similar results with more than a handful of other species so far.