In vitro characteristics of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and TLR7/8 ligand R848.

The quality of the separated fractions in sex-sorted semen is very important for the success of the artificial insemination. This study aimed to evaluate some in vitro characteristics (DNA quantity, kinematic parameters and enzymes activity) of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and toll-like receptors (TLR)7/8 ligand R848. The ejaculates from six rams were collected by artificial vagina and subjected to a computer-assisted semen analysis (CASA).

Clinical retrospective study of vaginal hyperplasia in the bitch (2012-2022).

A retrospective study was used to analyse canine vaginal hyperplasia ambulatory cases in Small animal clinic of the University Veterinary Hospital of the Faculty of Veterinary Medicine, Trakia University in Stara Zagora, Bulgaria between 2012 and 2022. The study was done to investigate the morbidity of vaginal hyperplasia in the bitch and its relationship with the breed, body weight, age, stage of the oestrous cycle, parity of dogs and grade of the condition. Grade II and grade III vaginal hyperplasia were observed in 3.

Influence of Ovarian Status and Steroid Hormone Concentration on Day of Timed Artificial Insemination (TAI) on the Reproductive Performance of Dairy Cows Inseminated with Sexed Semen.

This study aimed to evaluate the effect of the ovarian status and steroid hormone concentration on the day of TAI on the reproductive performance of dairy cows subjected to estrus synchronization treatment and timed artificial insemination with sexed semen. Seventy-eight cyclic Holstein cows pre-treated with PGF2α-GnRH were divided in two groups-I (Preselect-OvSynch, = 38) and II (OvSynch+PRID-7-day+eCG, = 40)-and inseminated with sexed semen. The presence of preovulatory follicle (PF) with or without corpus luteum (CL), the PF diameter, the estradiol (E) and progesterone (P) concentrations on the day of TAI, the pregnancy rate (PR) and embryo loss were determined.

Treatment of queens in estrus and after estrus with a GnRH-agonist implant containing 4.7 mg deslorelin; hormonal response, duration of efficacy, and reversibility.

Although slow release GnRH-agonist implants have been shown to effectively suppress the estrous cycle in queens, there are still several remaining questions about their use: if the probability and frequency of estrus induction because of initial stimulation is dependent on the stage of cycle when animals are treated, if all effects are reversible, and to what extent fertility is regained after the end of efficacy. The latter is of major interest to cat breeders who want temporary suppression of estrus in breeding animals. Twenty queens (14 with known fertility) were treated with a 4.

Treatment with Suprelorin in a pregnant cat.

Suppression of oestrus is of major interest in feral cat populations, but also in breeding queens temporarily not intended for breeding. Slow release gonadotropin-releasing hormone (GnRH) agonist implants are a new off-label approach for reproduction control in cats. However, initially, oestrus induction may occur and no data exist regarding what happens if previously mated queens are treated.

Ultrasonic evaluation of uterine involution in Bulgarian Murrah buffalo after administration of oxytocin.

The aim of this study was to determine the time taken for complete uterine involution in Bulgarian Murrah buffaloes following normal parturition and oxytocin stimulated milking; and to establish the time course of the change in size of the uterine horns, the cervix and caruncles between parturition and involution by means of ultrasonography. There were 17 animals in the study aged 3-6 years and average parity of 2.17 ± 0.