Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification.

A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on "biological nanoparticles" (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods.

The Photomodification Method Allows for Determining the Composition of the Full and Soft Protein Corona on the Lipid Surface of Composite Nanoparticles.

A protein corona (PC) is formed and maintained on the surface of any nanoparticle (NP) introduced into biological media. The full PC is formed by a hard and soft corona, and the latter determines the nature of the interaction of NPs with cells and the body's liquids. Nanomedicines are becoming increasingly important in modern health services, making information about the composition of PCs on the surface of NPs critically important for "managing" the behavior of nano-objects in the body.

Changes in the Ultrastructure of Cells Make It Possible to Identify and Analyze the Injuring Effects of Ciprofloxacin, Polycationic Amphiphile and Their Hybrid.

The purposeful development of synthetic antibacterial compounds requires an understanding of the relationship between effects of compounds and their chemical structure. This knowledge can be obtained by studying changes in bacteria ultrastructure under the action of antibacterial compounds of a certain chemical structure. Our study was aimed at examination of ultrastructural changes in cells caused by polycationic amphiphile based on 1,4‒diazabicyclo[2.

Isolation of Extracellular Vesicles of Holothuria (Sea Cucumber ).

Extracellular vesicles (EVs), carriers of molecular signals, are considered a critical link in maintaining homeostasis in mammals. Currently, there is growing interest in studying the role of EVs, including exosomes (subpopulation of EVs), in animals of other evolutionary levels, including marine invertebrates. We have studied the possibility of obtaining appropriate preparations of EVs from whole-body extract of holothuria using a standard combination of centrifugation and ultracentrifugation.

Changes in the Ultrastructure of Treated with Cationic Peptides and Chlorhexidine.

Antimicrobial peptides, including synthetic ones, are becoming increasingly important as a promising tool to fight multidrug-resistant bacteria. We examined the effect of cationic peptides HN-Arg-Phe-C(O)NH and HN-(Lys-Phe-Phe)-Lys-C(O)NH on , which remains one of the most harmful pathogens. Antiseptic chlorhexidine served as reference preparation.

Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids.

Use of multicellular spheroids in studies of nanoparticles (NPs) has increased in the last decade, however details of NPs interaction with spheroids are poorly known. We synthesized AuNPs (12.0 ± 0.

Changes in the Ultrastructure of Treated with Cationic Peptides.

is becoming increasingly harmful for humans, which determines the need for new effective antifungal preparations. Currently, when testing antifungals, various morphological methods are used, among which transmission electron microscopy (TEM) is not the leading one. In this work, we used TEM to study the submicroscopic changes in cells induced by cationic peptides R9F2 and (KFF)3K.