Publications by authors named "Anastasia Zhurikhina"
Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical limitations. Here, we report an optogenetic approach that utilizes an engineered Light-Regulated (LightR) allosteric switch module to achieve tight spatiotemporal control of enzymatic activity.
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- The increasing amount of imaging data in biomedical research necessitates the development of computational methods for automated extraction of quantitative information.
- A new approach is introduced that uses graph-based mapping of shape boundaries in images to facilitate the quantification and classification of complex biological forms.
- Demonstrated applications include identifying subtle differences in cellular structures, training models to understand multicellular network formation, and analyzing cellular responses to various chemical treatments.
At early stages of organismal development, endothelial cells self-organize into complex networks subsequently giving rise to mature blood vessels. The compromised collective behavior of endothelial cells leads to the development of a number of vascular diseases, many of which can be life-threatening. Cerebral cavernous malformation is an example of vascular diseases caused by abnormal development of blood vessels in the brain.
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- Developing molecular tools for Rho GTPase signaling helps understand cell function and cytoskeletal changes.
- The EdgeProps computational pipeline enables analysis of cell dynamics and protein activity through fluorescent signaling.
- It features an easy-to-use interface with three modules for processing, visualizing, and statistical analysis of single-cell imaging data.
Tip growth in fungi involves highly polarized secretion and modification of the cell wall at the growing tip. The genetic requirements for initiating polarized growth are perhaps best understood for the model budding yeast Saccharomyces cerevisiae. Once the cell is committed to enter the cell cycle by activation of G1 cyclin/cyclin-dependent kinase (CDK) complexes, the polarity regulator Cdc42 becomes concentrated at the presumptive bud site, actin cables are oriented toward that site, and septin filaments assemble into a ring around the polarity site.
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