Publications by authors named "Anastasia S Mikhailichenko"
Biology (Basel)
November 2022
Article Synopsis
- Yeast serves as a useful model for studying protein aggregation due to its ability to propagate amyloid prions, specifically the prion form of eRF3 (Sup35), which can disrupt translation termination.
- Sup35's N-terminal and middle domains are essential for maintaining its prion properties, and researchers often fuse these proteins to fluorescent markers for detection; however, not all fluorescent proteins are reliable for this purpose.
- The study found that while Sup35NM-mCherry showed diffuse fluorescence and was actively degraded, Sup35NM-TagRFP-T effectively detected prion aggregates, highlighting that the choice of fluorescent tags can significantly impact research outcomes and potentially lead to false negatives in detecting prion-bearing cells.
