A kinetic approach to the formation of two-mediator systems for developing microbial biosensors as exemplified by a rapid biochemical oxygen demand assay.

This work proposes a method of forming a microorganism-mediator(s) receptor system, in which the rates of separate stages of mediator bioelectrocatalysis are used as the basis for the development of biosensors for the biochemical oxygen demand (BOD) rapid assay. In the presence of a ferrocene mediator, the yeast was shown to enable oxidation of a larger range of substrates as compared with other investigated microorganisms-bacteria and yeast The rate constants of the interaction of the yeast with nine compounds, electron transfer mediators, were determined; the best mediator for these microorganisms was found to be neutral red (  = 0.681 ± 0.