Program freezing of diapausing embryos in the mouse.

Embryonal diapause is a characteristic feature of about 130 mammalian species. However, very few studies have addressed cryopreservation of diapausing embryos. This work is aimed to apply program freezing to blastocysts obtained from CD1 mice, which were at diapause state after ovariectomy and the subsequent hormonal therapy.

Raman spectroscopy of yeast cells cultured on a deuterated substrate.

Raman spectroscopy of cells cultured in a deuterated substrate is a promising approach to the characterization of mass transfer and enzymatic reactions in living cells. Here, we studied the potential of this approach using the example of yeast cells cultured under aerobic and anaerobic conditions. In our experiments, unadapted to DO Saccharomyces cerevisiae were cultured in a medium with different concentrations of deuterium oxide and deuterated glucose.