Publications by authors named "Anastasia D Sergeeva"
The lack of oxygen (O2) causes changes in the cell functioning. Modeling hypoxic conditions in vitro is challenging given that different cell types exhibit different sensitivities to tissue O2 levels. We present an effective in vivo platform for assessing various tissue and organ parameters in Danio rerio larvae under acute hypoxic conditions.
View Article and Find Full Text PDFIt is generally accepted that oxidative stress plays a key role in the development of ischemia-reperfusion injury in ischemic heart disease. However, the mechanisms how reactive oxygen species trigger cellular damage are not fully understood. Our study investigates redox state and highly reactive substances within neonatal and adult cardiomyocytes under hypoxia conditions.
View Article and Find Full Text PDFArticle Synopsis
- Diabetes increases the risk of ischemic stroke by worsening cerebral damage due to hyperglycemia, though the exact mechanisms are not fully understood.
- This study presents novel findings on the real-time dynamics of hydrogen peroxide (HO) in neuronal mitochondria during ischemic stroke, using advanced technology on both cultured cells and rat brains.
- Results indicate that high blood sugar doesn't impact HO generation in the ischemic area but does worsen the overall effects of the stroke, revealing how elevated glucose levels can alter mitochondrial function in neurons.
The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli.
View Article and Find Full Text PDF