[The trans-eyebrow supraorbital approach for removal of anterior cranial fossa and suprasellar meningiomas].

Unlabelled: Over the past two decades, improvements in surgical tools, navigation systems, and endoscopic techniques have resulted in the widespread use of keyhole surgery for a wide range of skull base tumors. Currently, the trans-eyebrow supraorbital approach is being increasingly used in surgery for anterior cranial fossa and parasellar tumors.

Material And Methods: The study included 7 patients who underwent surgery for meningioma using the trans-eyebrow supraorbital approach at the Burdenko Neurosurgical Institute in the period between 2013 and 2017.

[Use of the IntelliVent-ASV mode for maintaining the target EtCO2 range in patients with severe TBI].

Purpose: the study purpose was to evaluate the efficacy of the IntelliVent-ASV mode in maintaining the target range of PaCO2 in patients with severe TBI.

Material And Methods: The study included 12 severe TBI patients with the wakefulness level scored 4-9 (GCS). This was a crossover design study.

[INHOSPITAL TRANSPORT OF PATIENTS IN ACUTE PERIOD OF SEVERE ISOLATED TRAUMATIC BRAIN INJURY WITH MULTIMODAL MONITORING].

Unlabelled: Transporting patients out of intensive care unit (ICU) in the acute period of TBI to perform diagnostic tests is an integral part of the treatment process and may be associated with the risk of secondary brain injury. Despite the large number of studies related to in-hospital transport ofpatients with TBI there are no clear recommendations on the required monitoring.

Objective: To provide safe transportation of the patient by multimodal monitoring in acute period of brain injury.

Paradoxical air embolism resulted in acute myocardial infarction and massive ischemic brain injury in a patient operated on in a sitting position.

Paradoxical air embolism (PAE) is a rare life-threatening complication when air emboli enter arteries of the systemic circulation and cause their occlusion. Here, we describe a clinical case of PAE developed during neurosurgery in a patient in the sitting position. PAE led to injuries to the cerebral blood vessels, coronary arteries, and lungs, which caused death of the patient.

[Application of shot-latency cortical somato-sensory evoked potentials for evaluation of swallowing disorders in early period after posterior fossa surgery].

Unlabelled: Main tasks of early postoperative period in patients after posterior fossa surgery are the timely and safe weaning from mechanical ventilation and extubation. For clinical assessment of the hypopharynx function we use an algorithm developed in the intensive care unit (ICU) of the Burdenko Scientific Research Institute of Neurosurgery. Disadvantages of the clinical test for assessment of patient's readiness for extubation are subjectivity and impossibility to use it in conditions of even superficial sedation.

[Structural organization of C-hordein genes from barley].

Three nucleotide sequences from the barley genome, containing the C-hordein genes lambda CH1, lambda CH3, and lambda CH5, were cloned. They were shown to have different physical maps, and the structural organization of homologous sequences was found to be highly conservative. The surrounding sequences of C-hordein genes contain insertions specific in length and copy number.

[Nucleotide sequence of the barley C-hordein gene].

The nucleotide sequence of barley C-hordein gene lambda CH4 and its flanking regions of 2820 bp length was determined. The gene contains no introns and codes for 310 amino acid long polypeptide. The 94% of the deduced amino acid sequence of the mature protein (291 amino acids) is made up of a repeating octapeptide motiff, PQQPEPQQ, which is repeated throughout the peptide chain between a unique 12 amino acid long NH2 terminal and a unique 6 amino acid long COOH-terminal end.

[Pharmacokinetics of antipyrine, nifedipine and diazepam in experimental myocardial infarct].

It has been shown, that antipyrine, nifedipine, diazepam pharmacokinetics changes in different ways after myocardial infarction. On day 7, 14, and 21 after myocardial ischemia antipyrine T1/2 increased considerably, and antipyrine Cl and Kel decreased. Nifedipine T1/2 increased on day 7 only.

[The genomic organization and localization on the chromosomes of the HvRT family of DNA repetitive sequences in barley].

HvRT family of repetitive DNA sequences from barley genome appears to have complex hierarchical organization. Tandem repetition of 118-bp monomers constitutes lower level of HvRT-family organization. Amplification units of the higher level consist of several contiguous 118-bp monomers.

[Cloning and partial characteristics of the barley D-hordein sequence].

A number of clones containing major endosperm-specifically transcribed gene copies were selected from a cDNA library developed on the basis of barley endosperm mRNA. Approx. 30% of the recombinant clones carried sequences homologous to mRNA of various cereal storage proteins.

[The cloning of "residual" DNA and the determination of the primary structure for the fragments of the barley-specific family of tandem repetitive sequences].

High molecular weight "relic" DNA fraction can be separated from the bulk of barley DNA digested with different restriction enzymes by agarose gel electrophoresis. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. The clones representing HvRT family were sequenced.

[Nucleotide sequence of the B1-hordein gene of barley Hordeum vulgare L].

The gene encoding B1 hordein of Hordeum vulgare (cv. Donetsky 4) was cloned and entirely sequenced. It contains no introns and codes for of 293 amino acids long polypeptide with molecular weight 33418.

[Isolation of DNA fragments of the barley genome, supporting autonomous regulation of plasmids in Saccharomyces cerevisiae].

BamHI fragments of the barley genomic DNA were cloned in Escherichia coli cells on the vector plasmid YIp5 carrying the URA3 gene of Saccharomyces cerevisiae. Yeast cells were transformed by individual plasmid DNA preparations from each clone selected. Approximately 10% of the studied plasmids are able to replicate in yeast cells.

[Organization and primary nucleotide sequence of 5S rRNA genes in barley Hordeum vulgare].

A BamHI DNA fragment of 301 bp corresponding to the main repeating unit of 5S rRNA was isolated from barley genomic DNA. The primary nucleotide sequence of this fragment was determined and a high level of homology was found between coding sequences of 5S rRNA genes of barley, wheat and rye. At the same time, spacer's nucleotide sequences of different species of cereals were changed dramatically.

[A new class of mobile genetic elements of Drosophila melanogaster].

Clone Dm A89 was obtained upon cloning of DNA fragments coding abundant poly(A+)RNA's of D. melanogaster. Dm A89 was identified as a new transposable element using in situ hybridization with polytene chromosomes of two independent highly isogenic lines of D.

[Effect of diucifon and its structural precursors on inflammatory and pain reactions].

The anti-inflammatory and analgesic action of diucifon, methyluracil and 4,4'-diaminodiphenylsulfone (DDS) was studied in comparison with some nonsteroid preparations. In three traditional models of agar, kaolin and carrageenan paw edema and in the models of analgesia (convulsions, induced by intraabdominal administration of acetic acid to mice and hyperalgesia according to Randall-Selitto's test in rats), diucifon proved more active than its precursors. In Randall-Selitto's test the efficiency of diucifon was 1.

[Visualization of elementary structures in polytene chromosomes of Drosophila melanogaster].

Electron microscopic study of total polytene chromosome preparations makes it possible to reveal elementary structures of polytene chromosomes. Elementary chromosomes are detected which contain chromomeric and interchromomeric regions. Transcription is seen in puffs and in many bands.

[Molecular approaches to the systematic mapping of the human genome].

A review of current progress in human gene mapping methods is presented. The advantages and restrictions of several mapping methods are discussed. The main bulk of the review is concerned with perspectives of using special collection of "molecular-genetic markers" (MGM).

[Transcription of portions of chromosomes corresponding to regions of intercalary heterochromatin in Drosophila melanogaster cell cultures].

Labelled RNA preparations (total newly synthesized RNA, as well as stable cytoplasmic RNA) isolated from a cell culture of Drosophila melanogaster were hybridized in situ with polytene chromosomes. Apart from the nucleolus, in all cases the regions adjacent to he chromocentre in the polytene chromosomes and the intercalary heterochromatin regions in the X chromosome and the autosomes are the most intensively labelled. In the case of asynapsis of polytene chromosomes in heterozygotes the label is detected in a number of intercalary heterochromatin sites in one homologous only ("the asymmetrical label").

A new type of organization of the genetic material in eukaryotes.

An investigation of the properties of a number of genes of Drosophila, obtained by cloning recombinant DNAs, led to the detection of a new type of organization of genetic material. It was found that a number of actively working structural genes of Drosophila are represented by a large number of copies, scattered over its chromosomes. Their localization in the chromosomes is variable, although they are always detected in regions of intercalary heterochromatin.

[Rate of DNA synthesis and the size of replication units in cell culture of Drosophila melanogaster].

The rate of DNA replication and the size of replication units was examined by means of pulse-labeling with 3H-thymidine and DNA autoradiography in the chromsomes of Drosophila melanogaster synchronized cells culture in vitro. The center-to-center distance between adjacent labeled section of DNA fibers was taken for a size of replication unit, i.e.

[DNA content, replication and transcription rate in different site of the X-chromosome of Drosophila melanogaster].

Duration of replication period and relative content of DNA and chromosomal newly-synthesized RNA were determined in nine sites of the region 1A1-3C8 in the polytene X-chromosome of D. melanogaster. Determination of DNA content by cytophotometry of Feulgen stained preparations and by autoradiography after long-term [3H]thymidine labeling gave similar results for all nine sites.